Human BMP-6 ELISA Kit PicoKine®

Human BMP-6 ELISA Kit PicoKine® (96 Tests) . Quantitate Human BMP-6 in Bone tissue, cell culture supernatants, serum. Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, BMP-6 has a proposed role in early development. In addition, the fact that this BMP is closely related to BMP5 and BMP7 has lead to speculation of possible bone inductive activity.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 294 250 237 176 239 17.39 7.3% Sample 2 957 1071 863 988 970 29.87 3.1% Sample 3 4433 2670 4949 4508 4140 282.76 6.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 294 250 237 176 239 17.39 7.3% Sample 1 294 250 237 176 239 17.39 7.3% Sample 2 957 1071 863 988 970 29.87 3.1% Sample 2 957 1071 863 988 970 29.87 3.1% Sample 3 4433 2670 4949 4508 4140 282.76 6.8% Sample 3 4433 2670 4949 4508 4140 282.76 6.8%

Synonyms

Bone morphogenetic protein 6; BMP-6; VG-1-related protein; VGR-1; Bmp6; Bmp-6, Vgr

Gene Name

BMP6

Gene ID

654

UniProt

P22004

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Applications

ELISA

Field of Research

Cell Biology, Cell Cycle, Cell Differentiation, Cytoskeleton/ECM, Extracellular Matrix, Growth Factors/Hormones, Secreted, Signal Transduction, Signaling Pathways, Stem Cells, Structures, TGF Beta

Standard

10 ng/Tube

Sample Type

Bone tissue, cell culture supernatants, serum

Detection Range

156.0-10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 294 957 4433 205 925 4419 Standard deviation 11.26 48.33 321.84 4.45 38.57 90.15 CV (%) 3.8% 5.1% 7.3% 2.2% 4.2% 2.0% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 294 957 4433 205 925 4419 Standard deviation 11.26 48.33 321.84 4.45 38.57 90.15 CV (%) 3.8% 5.1% 7.3% 2.2% 4.2% 2.0% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 294 957 4433 205 925 4419 Mean (pg/ml) 294 957 4433 205 925 4419 Standard deviation 11.26 48.33 321.84 4.45 38.57 90.15 Standard deviation 11.26 48.33 321.84 4.45 38.57 90.15 CV (%) 3.8% 5.1% 7.3% 2.2% 4.2% 2.0% CV (%) 3.8% 5.1% 7.3% 2.2% 4.2% 2.0%

Sensitivity

<20 pg/mL

Function

Induces cartilage and bone formation. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

57226 MW

Specificity

Natural and recombinant human BMP-6

Gene Name Synonym

Bone morphogenetic protein 6

Subcellular Location

Secreted .

Sample Data

Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.045 0.089 0.127 0.211 0.393 0.678 1.323 2.204 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.045 0.089 0.127 0.211 0.393 0.678 1.323 2.204 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.045 0.089 0.127 0.211 0.393 0.678 1.323 2.204 O.D. 0.045 0.089 0.127 0.211 0.393 0.678 1.323 2.204