Human BMP-6 ELISA Kit PicoKine®

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, BMP-6 has a proposed role in early development. In addition, the fact that this BMP is closely related to BMP5 and BMP7 has lead to speculation of possible bone inductive activity.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 294 250 237 176 239 17.39 7.3% Sample 2 957 1071 863 988 970 29.87 3.1% Sample 3 4433 2670 4949 4508 4140 282.76 6.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 294 250 237 176 239 17.39 7.3% Sample 1 294 250 237 176 239 17.39 7.3% Sample 2 957 1071 863 988 970 29.87 3.1% Sample 2 957 1071 863 988 970 29.87 3.1% Sample 3 4433 2670 4949 4508 4140 282.76 6.8% Sample 3 4433 2670 4949 4508 4140 282.76 6.8%

Cell Biology, Cell Cycle, Cell Differentiation, Cytoskeleton/ECM, Extracellular Matrix, Growth Factors/Hormones, Secreted, Signal Transduction, Signaling Pathways, Stem Cells, Structures, TGF Beta

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 294 957 4433 205 925 4419 Standard deviation 11.26 48.33 321.84 4.45 38.57 90.15 CV (%) 3.8% 5.1% 7.3% 2.2% 4.2% 2.0% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 294 957 4433 205 925 4419 Standard deviation 11.26 48.33 321.84 4.45 38.57 90.15 CV (%) 3.8% 5.1% 7.3% 2.2% 4.2% 2.0% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 294 957 4433 205 925 4419 Mean (pg/ml) 294 957 4433 205 925 4419 Standard deviation 11.26 48.33 321.84 4.45 38.57 90.15 Standard deviation 11.26 48.33 321.84 4.45 38.57 90.15 CV (%) 3.8% 5.1% 7.3% 2.2% 4.2% 2.0% CV (%) 3.8% 5.1% 7.3% 2.2% 4.2% 2.0%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.045 0.089 0.127 0.211 0.393 0.678 1.323 2.204 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.045 0.089 0.127 0.211 0.393 0.678 1.323 2.204 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.045 0.089 0.127 0.211 0.393 0.678 1.323 2.204 O.D. 0.045 0.089 0.127 0.211 0.393 0.678 1.323 2.204