Human CXCL2/GRO beta ELISA Kit PicoKine®
Human CXCL2/GRO beta ELISA Kit PicoKine® (96 Tests) . Quantitate Human CXCL2/GRO beta in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Product Specifications
Background
Chemokine (C-X-C motif) ligand 2 (CXCL2) is a small cytokine belonging to the CXC chemokine family that is also called macrophage inflammatory protein 2-alpha (MIP2-alpha), Growth-regulated protein beta (Gro-beta) and Gro oncogene-2 (Gro-2) . CXCL2 is 90% identical in amino acid sequence as a related chemokine, CXCL1. This chemokine is secreted by monocytes and macrophages and is chemotactic for polymorphonuclear leukocytes and hematopoietic stem cells. The gene for CXCL2 is located on human chromosome 4 in a cluster of other CXC chemokines. CXCL2 mobilizes cells by interacting with a cell surface chemokine receptor called CXCR2.
Anticoagulant
Heparin or EDTA
Reproducibility
Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 26 17 19 22 21 1.42 6.8% Sample 2 78 99 82 94 88 5.64 6.4% Sample 3 325 253 464 470 378 15.8 4.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 26 17 19 22 21 1.42 6.8% Sample 1 26 17 19 22 21 1.42 6.8% Sample 2 78 99 82 94 88 5.64 6.4% Sample 2 78 99 82 94 88 5.64 6.4% Sample 3 325 253 464 470 378 15.8 4.2% Sample 3 325 253 464 470 378 15.8 4.2%
Synonyms
C-X-C motif chemokine 2; Growth-regulated protein beta; Gro-beta; Macrophage inflammatory protein 2-alpha; MIP2-alpha; GRO-beta (5-73) ; GRO-beta-T; Hematopoietic synergistic factor; HSF; SB-251353; CXCL2; GRO2, GROB, MIP2A, SCYB2
Gene Name
CXCL2
Gene ID
2920
UniProt
P19875
Reactivity
Human
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Capture Antibody
Monoclonal antibody from goat
Detection Antibody
Monoclonal antibody from goat
Tissue Specificity
Predominantly expressed in osteoclasts (bones) .
Applications
ELISA
Field of Research
Chemokines, Immunology, Innate Immunity
Standard
10 ng/Tube
Sample Type
Cell culture supernatants, serum, plasma (heparin, EDTA)
Detection Range
15.6-500 pg/mL
Precision
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 26 78 325 30 120 376 Standard deviation 0.63 5.73 22.2 1.71 8.39 18.54 CV (%) 2.4% 7.4% 6.8% 5.7% 7.0% 4.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 26 78 325 30 120 376 Standard deviation 0.63 5.73 22.2 1.71 8.39 18.54 CV (%) 2.4% 7.4% 6.8% 5.7% 7.0% 4.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 26 78 325 30 120 376 Mean (pg/ml) 26 78 325 30 120 376 Standard deviation 0.63 5.73 22.2 1.71 8.39 18.54 Standard deviation 0.63 5.73 22.2 1.71 8.39 18.54 CV (%) 2.4% 7.4% 6.8% 5.7% 7.0% 4.9% CV (%) 2.4% 7.4% 6.8% 5.7% 7.0% 4.9%
Sensitivity
<5 pg/mL
Function
Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta (5-73) shows a highly enhanced hematopoietic activity. .
Components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
Storage Conditions
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Product Datasheet
https://www.bosterbio.com/datasheet?sku=EK1461
Product MSDS
https://www.bosterbio.com/msds?sku=EK1461
Calculated Molecular Weight
11389 MW
Specificity
Natural and recombinant human CXCL2
Gene Name Synonym
C-X-C motif chemokine 2
Subcellular Location
Secreted.
Protein Name
C-X-C motif chemokine 2
Sample Data
Concentration (pg/ml) 0 15.6 31.22 62.5 125 250 500 O.D. 0.025 0.114 0.212 0.41 0.785 1.384 2.211 nan Concentration (pg/ml) 0 15.6 31.22 62.5 125 250 500 O.D. 0.025 0.114 0.212 0.41 0.785 1.384 2.211 nan Concentration (pg/ml) 0 15.6 31.22 62.5 125 250 500 Concentration (pg/ml) 0 15.6 31.22 62.5 125 250 500 O.D. 0.025 0.114 0.212 0.41 0.785 1.384 2.211 nan O.D. 0.025 0.114 0.212 0.41 0.785 1.384 2.211 nan
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