Human CXCL2/GRO beta ELISA Kit PicoKine®

Human CXCL2/GRO beta ELISA Kit PicoKine® (96 Tests) . Quantitate Human CXCL2/GRO beta in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chemokine (C-X-C motif) ligand 2  (CXCL2) is a small  cytokine  belonging to the CXC  chemokine  family that is also called  macrophage inflammatory protein 2-alpha  (MIP2-alpha),   Growth-regulated protein beta  (Gro-beta) and  Gro oncogene-2 (Gro-2) . CXCL2 is 90% identical in  amino acid  sequence as a related chemokine,   CXCL1. This chemokine is secreted by monocytes  and  macrophages  and is  chemotactic  for  polymorphonuclear leukocytes  and  hematopoietic stem cells. The gene for CXCL2 is located on human  chromosome 4  in a cluster of other CXC chemokines. CXCL2 mobilizes cells by interacting with a cell surface  chemokine receptor  called  CXCR2.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 26 17 19 22 21 1.42 6.8% Sample 2 78 99 82 94 88 5.64 6.4% Sample 3 325 253 464 470 378 15.8 4.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 26 17 19 22 21 1.42 6.8% Sample 1 26 17 19 22 21 1.42 6.8% Sample 2 78 99 82 94 88 5.64 6.4% Sample 2 78 99 82 94 88 5.64 6.4% Sample 3 325 253 464 470 378 15.8 4.2% Sample 3 325 253 464 470 378 15.8 4.2%

Synonyms

C-X-C motif chemokine 2; Growth-regulated protein beta; Gro-beta; Macrophage inflammatory protein 2-alpha; MIP2-alpha; GRO-beta (5-73) ; GRO-beta-T; Hematopoietic synergistic factor; HSF; SB-251353; CXCL2; GRO2, GROB, MIP2A, SCYB2

Gene Name

CXCL2

Gene ID

2920

UniProt

P19875

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Tissue Specificity

Predominantly expressed in osteoclasts (bones) .

Applications

ELISA

Field of Research

Chemokines, Immunology, Innate Immunity

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

15.6-500 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 26 78 325 30 120 376 Standard deviation 0.63 5.73 22.2 1.71 8.39 18.54 CV (%) 2.4% 7.4% 6.8% 5.7% 7.0% 4.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 26 78 325 30 120 376 Standard deviation 0.63 5.73 22.2 1.71 8.39 18.54 CV (%) 2.4% 7.4% 6.8% 5.7% 7.0% 4.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 26 78 325 30 120 376 Mean (pg/ml) 26 78 325 30 120 376 Standard deviation 0.63 5.73 22.2 1.71 8.39 18.54 Standard deviation 0.63 5.73 22.2 1.71 8.39 18.54 CV (%) 2.4% 7.4% 6.8% 5.7% 7.0% 4.9% CV (%) 2.4% 7.4% 6.8% 5.7% 7.0% 4.9%

Sensitivity

<5 pg/mL

Function

Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta (5-73) shows a highly enhanced hematopoietic activity. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

11389 MW

Specificity

Natural and recombinant human CXCL2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

C-X-C motif chemokine 2

Subcellular Location

Secreted.

Protein Name

C-X-C motif chemokine 2

Sample Data

Concentration (pg/ml) 0 15.6 31.22 62.5 125 250 500 O.D. 0.025 0.114 0.212 0.41 0.785 1.384 2.211 nan Concentration (pg/ml) 0 15.6 31.22 62.5 125 250 500 O.D. 0.025 0.114 0.212 0.41 0.785 1.384 2.211 nan Concentration (pg/ml) 0 15.6 31.22 62.5 125 250 500 Concentration (pg/ml) 0 15.6 31.22 62.5 125 250 500 O.D. 0.025 0.114 0.212 0.41 0.785 1.384 2.211 nan O.D. 0.025 0.114 0.212 0.41 0.785 1.384 2.211 nan