Mouse IL-2 High Sensitivity ELISA Kit PicoKine®

Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. The human gene for interleukin 2 (IL2) is assigned to chromosome 4. Mouse IL-2 contains 149 amino acids in mature form with the molecular mass of 17.2KDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1 1 1 1 1 0.05 4.8% Sample 2 2 2 2 2 2 0.13 6.7% Sample 3 8 5 7 6 7 0.44 6.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1 1 1 1 1 0.05 4.8% Sample 1 1 1 1 1 1 0.05 4.8% Sample 2 2 2 2 2 2 0.13 6.7% Sample 2 2 2 2 2 2 0.13 6.7% Sample 3 8 5 7 6 7 0.44 6.7% Sample 3 8 5 7 6 7 0.44 6.7%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1 2 8 1 2 5 Standard deviation 0.06 0.09 0.55 0.02 0.1 0.22 CV (%) 6.2% 4.4% 6.9% 2.5% 4.9% 4.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1 2 8 1 2 5 Standard deviation 0.06 0.09 0.55 0.02 0.1 0.22 CV (%) 6.2% 4.4% 6.9% 2.5% 4.9% 4.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1 2 8 1 2 5 Mean (pg/ml) 1 2 8 1 2 5 Standard deviation 0.06 0.09 0.55 0.02 0.1 0.22 Standard deviation 0.06 0.09 0.55 0.02 0.1 0.22 CV (%) 6.2% 4.4% 6.9% 2.5% 4.9% 4.3% CV (%) 6.2% 4.4% 6.9% 2.5% 4.9% 4.3%

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine- activated killer cells, natural killer cells, and glioma cells.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 0.3 0.63 1.25 2.5 5 10 20 O.D. 0.053 0.126 0.188 0.301 0.536 0.973 1.747 2.88 Concentration (pg/ml) 0 0.3 0.63 1.25 2.5 5 10 20 O.D. 0.053 0.126 0.188 0.301 0.536 0.973 1.747 2.88 Concentration (pg/ml) 0 0.3 0.63 1.25 2.5 5 10 20 Concentration (pg/ml) 0 0.3 0.63 1.25 2.5 5 10 20 O.D. 0.053 0.126 0.188 0.301 0.536 0.973 1.747 2.88 O.D. 0.053 0.126 0.188 0.301 0.536 0.973 1.747 2.88

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.