Mouse IL-17 High Sensitivity ELISA Kit PicoKine®

IL-17 is an inflammatory cytokine produced primarily by a unique lineage of CD4 T cells that plays critical roles in the pathogenesis of multiple autoimmune diseases. Interleukin-17 is expressed by activated T cells and is 57% identical to the 17- to 26-kD secretory glycoprotein encoded by gene 13 of the herpesvirus saimiri (HVS-13) . IL17 induces nuclear factor kappa-B and the expression of IL6, intercellular adhesion molecule-1, granulocyte macrophage colony-stimulating factor, and prostaglandin E2, as well as the maturation of CD34 positive hematopoietic precursors into neutrophils. Anti-IL17 antibodies significantly inhibited osteoclast formation induced by culture media of RA synovial tissues. The standard product used in this kit is recombinant mouse IL-17, consisting of 137 amino acids with the molecular mass of 15.5KDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1 1 1 1 1 0.04 3.9% Sample 2 4 4 5 4 4 0.24 5.7% Sample 3 18 16 19 17 18 0.91 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1 1 1 1 1 0.04 3.9% Sample 1 1 1 1 1 1 0.04 3.9% Sample 2 4 4 5 4 4 0.24 5.7% Sample 2 4 4 5 4 4 0.24 5.7% Sample 3 18 16 19 17 18 0.91 5.2% Sample 3 18 16 19 17 18 0.91 5.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1 4 18 1 4 20 Standard deviation 0.02 0.30 1.31 0.07 0.09 1.10 CV (%) 2.1% 7.4% 7.3% 7.2% 2.4% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1 4 18 1 4 20 Standard deviation 0.02 0.30 1.31 0.07 0.09 1.10 CV (%) 2.1% 7.4% 7.3% 7.2% 2.4% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1 4 18 1 4 20 Mean (pg/ml) 1 4 18 1 4 20 Standard deviation 0.02 0.30 1.31 0.07 0.09 1.10 Standard deviation 0.02 0.30 1.31 0.07 0.09 1.10 CV (%) 2.1% 7.4% 7.3% 7.2% 2.4% 5.5% CV (%) 2.1% 7.4% 7.3% 7.2% 2.4% 5.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.047 0.108 0.165 0.281 0.467 0.795 1.471 2.356 Concentration (pg/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.047 0.108 0.165 0.281 0.467 0.795 1.471 2.356 Concentration (pg/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (pg/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.047 0.108 0.165 0.281 0.467 0.795 1.471 2.356 O.D. 0.047 0.108 0.165 0.281 0.467 0.795 1.471 2.356

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.