Mouse IL-1 Beta High Sensitivity ELISA Kit PicoKine®

Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone1. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB) -regulated genes2. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells3. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture4. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 351 346 324 358 344 12.71 3.6% Sample 2 1479 1733 1681 1766 1664 111.44 6.6% Sample 3 5183 4874 5083 5078 5054 112.31 2.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 351 346 324 358 344 12.71 3.6% Sample 1 351 346 324 358 344 12.71 3.6% Sample 2 1479 1733 1681 1766 1664 111.44 6.6% Sample 2 1479 1733 1681 1766 1664 111.44 6.6% Sample 3 5183 4874 5083 5078 5054 112.31 2.2% Sample 3 5183 4874 5083 5078 5054 112.31 2.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 3 9 44 2 9 25 Standard deviation 0.18 0.50 1.17 0.12 0.55 1.27 CV (%) 6.1% 5.5% 2.7% 6.0% 6.1% 5.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 3 9 44 2 9 25 Standard deviation 0.18 0.50 1.17 0.12 0.55 1.27 CV (%) 6.1% 5.5% 2.7% 6.0% 6.1% 5.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 3 9 44 2 9 25 Mean (pg/ml) 3 9 44 2 9 25 Standard deviation 0.18 0.50 1.17 0.12 0.55 1.27 Standard deviation 0.18 0.50 1.17 0.12 0.55 1.27 CV (%) 6.1% 5.5% 2.7% 6.0% 6.1% 5.1% CV (%) 6.1% 5.5% 2.7% 6.0% 6.1% 5.1%

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

Concentration (pg/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.071 0.136 0.175 0.271 0.428 0.752 1.279 2.047 Concentration (pg/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.071 0.136 0.175 0.271 0.428 0.752 1.279 2.047 Concentration (pg/ml) 0 1.56 3.12 6.25 12.5 25 50 100 Concentration (pg/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.071 0.136 0.175 0.271 0.428 0.752 1.279 2.047 O.D. 0.071 0.136 0.175 0.271 0.428 0.752 1.279 2.047

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.