Human IL-6 High Sensitivity ELISA Kit PicoKine®

The human interferon-beta 2 gene (IFNB2) product is identical to that for the B-cell stimulation factor-2 (BSF-2), the hybridoma growth factor (HGF) (interleukin-6), and the hepatocyte stimulating factor (HSF) . Proteins derived from this gene mediate the plasma protein response to tissue injury (acute-phase response) and regulate the growth and differentiation of both B and T cells. Interleukin-6 (IL6) has come to be regarded as a potential osteoporotic factor because it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency. IL-6 has many roles essential to the regulation of the immune response, hematopoiesis, and bone resorption. It is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. Overproduction of IL-6, a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline. BSF-2 is a novel interleukin consisting of 184 amino acids. The standard product used in this kit is recombinant human IL-6, consisting of 184 amino acids with the molecular mass of 20.3kDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1 1 1 1 1 0.081 7.6% Sample 2 6 6 6 5 6 0.35 6.1% Sample 3 13 20 21 24 20 0.57 2.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1 1 1 1 1 0.081 7.6% Sample 1 1 1 1 1 1 0.081 7.6% Sample 2 6 6 6 5 6 0.35 6.1% Sample 2 6 6 6 5 6 0.35 6.1% Sample 3 13 20 21 24 20 0.57 2.9% Sample 3 13 20 21 24 20 0.57 2.9%

Interleukin-6; IL-6; B-cell stimulatory factor 2; BSF-2; CTL differentiation factor; CDF; Hybridoma growth factor; Interferon beta-2; IFN-beta-2; IL6; IFNB2

Angiogenesis, Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Invasion/Microenvironment, Metabolism, Neurology Process, Neuroscience, Obesity, Tumor Immunology

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1 5 16 1 4 17 Standard deviation 0.07 0.14 0.65 0.06 0.13 1.03 CV (%) 7.3% 2.9% 4.1% 5.6% 3.4% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1 5 16 1 4 17 Standard deviation 0.07 0.14 0.65 0.06 0.13 1.03 CV (%) 7.3% 2.9% 4.1% 5.6% 3.4% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1 5 16 1 4 17 Mean (pg/ml) 1 5 16 1 4 17 Standard deviation 0.07 0.14 0.65 0.06 0.13 1.03 Standard deviation 0.07 0.14 0.65 0.06 0.13 1.03 CV (%) 7.3% 2.9% 4.1% 5.6% 3.4% 6.1% CV (%) 7.3% 2.9% 4.1% 5.6% 3.4% 6.1%

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T (H) 17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.056 0.125 0.151 0.233 0.321 0.618 1.12 1.707 Concentration (pg/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.056 0.125 0.151 0.233 0.321 0.618 1.12 1.707 Concentration (pg/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (pg/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.056 0.125 0.151 0.233 0.321 0.618 1.12 1.707 O.D. 0.056 0.125 0.151 0.233 0.321 0.618 1.12 1.707

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.