Mouse TNF Alpha High Sensitivity ELISA Kit PicoKine®

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 2 2 2 3 2 0.06 2.5% Sample 2 10 8 8 12 10 0.71 7.5% Sample 3 42 43 36 46 42 1.03 2.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 2 2 2 3 2 0.06 2.5% Sample 1 2 2 2 3 2 0.06 2.5% Sample 2 10 8 8 12 10 0.71 7.5% Sample 2 10 8 8 12 10 0.71 7.5% Sample 3 42 43 36 46 42 1.03 2.5% Sample 3 42 43 36 46 42 1.03 2.5%

Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Tumor necrosis factor, membrane form; N-terminal fragment; NTF; Intracellular domain 1; ICD1; Intracellular domain 2; ICD2; C-domain 1; C-domain 2; Tumor necrosis factor, soluble form; Tnf; Tnfa, Tnfsf2

Metabolism, Signal Transduction, Immunology, Cancer, Atherosclerosis, Cardiovascular, Growth Factors/Hormones, Growth Factors, Innate Immunity, Vascular Inflammation, Cytokines, TNF Superfamily

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2 10 42 3 10 34 Standard deviation 0.10 0.44 1.97 0.21 0.40 1.28 CV (%) 5.0% 4.4% 4.7% 7.0% 4.0% 3.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2 10 42 3 10 34 Standard deviation 0.10 0.44 1.97 0.21 0.40 1.28 CV (%) 5.0% 4.4% 4.7% 7.0% 4.0% 3.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 2 10 42 3 10 34 Mean (pg/ml) 2 10 42 3 10 34 Standard deviation 0.10 0.44 1.97 0.21 0.40 1.28 Standard deviation 0.10 0.44 1.97 0.21 0.40 1.28 CV (%) 5.0% 4.4% 4.7% 7.0% 4.0% 3.8% CV (%) 5.0% 4.4% 4.7% 7.0% 4.0% 3.8%

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.074 0.156 0.215 0.349 0.618 1.026 1.676 2.338 Concentration (pg/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.074 0.156 0.215 0.349 0.618 1.026 1.676 2.338 Concentration (pg/ml) 0 1.56 3.12 6.25 12.5 25 50 100 Concentration (pg/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.074 0.156 0.215 0.349 0.618 1.026 1.676 2.338 O.D. 0.074 0.156 0.215 0.349 0.618 1.026 1.676 2.338

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.