Human IFN Gamma High Sensitivity ELISA Kit PicoKine®

Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1 1 1 1 1 0.03 2.7% Sample 2 4 4 6 5 5 0.32 6.7% Sample 3 22 16 22 13 18 0.48 2.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1 1 1 1 1 0.03 2.7% Sample 1 1 1 1 1 1 0.03 2.7% Sample 2 4 4 6 5 5 0.32 6.7% Sample 2 4 4 6 5 5 0.32 6.7% Sample 3 22 16 22 13 18 0.48 2.6% Sample 3 22 16 22 13 18 0.48 2.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1 4 22 1 5 17 Standard deviation 0.04 0.21 1.25 0.05 0.18 0.54 CV (%) 3.7% 5.2% 5.7% 4.9% 3.7% 3.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1 4 22 1 5 17 Standard deviation 0.04 0.21 1.25 0.05 0.18 0.54 CV (%) 3.7% 5.2% 5.7% 4.9% 3.7% 3.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1 4 22 1 5 17 Mean (pg/ml) 1 4 22 1 5 17 Standard deviation 0.04 0.21 1.25 0.05 0.18 0.54 Standard deviation 0.04 0.21 1.25 0.05 0.18 0.54 CV (%) 3.7% 5.2% 5.7% 4.9% 3.7% 3.2% CV (%) 3.7% 5.2% 5.7% 4.9% 3.7% 3.2%

Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.012 0.07 0.115 0.216 0.356 0.647 1.168 2.023 Concentration (pg/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.012 0.07 0.115 0.216 0.356 0.647 1.168 2.023 Concentration (pg/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (pg/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.012 0.07 0.115 0.216 0.356 0.647 1.168 2.023 O.D. 0.012 0.07 0.115 0.216 0.356 0.647 1.168 2.023

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.