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Anti-PAM Antibody Picoband®

Boster Bio Anti-PAM Antibody Picoband® catalog # A00332-2. Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

This gene encodes a multifunctional protein. The encoded preproprotein is proteolytically processed to generate the mature enzyme. This enzyme includes two domains with distinct catalytic activities, a peptidylglycine alpha-hydroxylating monooxygenase (PHM) domain and a peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL) domain. These catalytic domains work sequentially to catalyze the conversion of neuroendocrine peptides to active alpha-amidated products. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.

Synonyms

PAM; Peptidyl-glycine alpha-amidating monooxygenase; PAM [Includes: Peptidylglycine alpha-hydroxylating monooxygenase; PHM; EC 1.14.17.3; Peptidyl-alpha-hydroxyglycine alpha-amidating lyase; EC 4.3.2.5; Peptidylamidoglycolate lyase; PAL]

Gene Name

PAM

Gene ID

5066

UniProt

P19021

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human PAM recombinant protein (Position: K69-R914) . Human PAM shares 97.4% amino acid (aa) sequence identity with mouse PAM.

Clonality

Polyclonal

Applications

WB, IHC, Flow Cytometry, ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

References & Citations

1. Eipper, B. A., Green, C. B.-R., Campbell, T. A., Stoffers, D. A., Keutmann, H. T., Mains, R. E., Ouafik, L.'H. Alternative splicing and endoproteolytic processing generate tissue-specific forms of pituitary peptidylglycine alpha-amidating monooxygenase (PAM) . J. Biol. Chem. 267: 4008-4015, 1992. 2. Glauder, J., Ragg, H., Rauch, J., Engels, J. W. Human peptidylglycine alpha-amidating monooxygenase: cDNA, cloning and functional expression of a truncated form in COS cells. Biochem. Biophys. Res. Commun. 169: 551-558, 1990. 3. Gross, M. B. Personal Communication. Baltimore, Md. 5/17/2021.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

108 kDa

Observed Molecular Weight

120 kDa

Applications Notes

6

Gene Name Synonym

Peptidylglycine alpha-amidating monooxygenase

Protein Name

Peptidyl-glycine alpha-amidating monooxygenase

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

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