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Anti-NF-M/NEFM Antibody Picoband®

Boster Bio Anti-NF-M/NEFM Antibody Picoband® catalog # A06821-2. Tested in WB, IHC, IF, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Synonyms

NEFM; NEF3; NFM; Neurofilament medium polypeptide; NF-M; 160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein

Gene Name

NEFM

Gene ID

4741

UniProt

P07197

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human NF-M/NEFM recombinant protein (Position: Q125-D916) . Human NF-M/NEFMshares 72.3% and 72% amino acid (aa) sequence identity with mouse and rat NF-M/NEFM, respectively.

Clonality

Polyclonal

Applications

WB, IHC, IF, IP, Flow Cytometry, ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

References & Citations

1. Hartz, P. A. Personal Communication. Baltimore, Md. 5/21/2014. 2. Levy, E., Liem, R. K. H., D'Eustachio, P., Cowan, N. J. Structure and evolutionary origin of the gene encoding NF-M, the middle-molecular-mass neurofilament protein. Europ. J. Biochem. 166: 71-77, 1987. 3. Myers, M. W., Lazzarini, R. A., Lee, V. M.-Y., Schlaepfer, W. W., Nelson, D. L. The human mid-size neurofilament subunit: a repeated protein sequence and the relationship of its gene to the intermediate filament gene family. EMBO J. 6: 1617-1626, 1987.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

102 kDa

Observed Molecular Weight

160 kDa

Applications Notes

6

Gene Name Synonym

Neurofilament medium chain

Protein Name

Neurofilament medium polypeptide

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

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