Anti-GTF2A2 Antibody Picoband®

Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and the general initiation factors TFIIA, TFIIB (MIM 189963), TFIID (MIM 313650), TFIIE (MIM 189962), TFIIF (MIM 189968), TFIIG/TFIIJ, and TFIIH (MIM 189972) . The first step involves recognition of the TATA element by the TATA-binding subunit (TBP; MIM 600075) and may be regulated by TFIIA, a factor that interacts with both TBP and a TBP-associated factor (TAF; MIM 600475) in TFIID. TFIIA has 2 subunits (43 and 12 kD) in yeast and 3 subunits in higher eukaryotes. In HeLa extracts, it consists of a 35-kD alpha subunit and a 19-kD beta subunit encoded by the N- and C-terminal regions of GTF2A1 (MIM 600520), respectively, and a 12-kD gamma subunit encoded by GTF2A2 (DeJong et al., 1995 [PubMed 7724559]) .

GTF2A2; TF2A2; Transcription initiation factor IIA subunit 2; General transcription factor IIA subunit 2; TFIIA p12 subunit; TFIIA-12; TFIIAS; Transcription initiation factor IIA gamma chain; TFIIA-gamma

E.coli-derived human GTF2A2 recombinant protein (Position: M1-E109) . Human GTF2A2shares 99.1% amino acid (aa) sequence identity with both mouse and rat GTF2A2.

1. DeJong, J., Bernstein, R., Roeder, R. G. Human general transcription factor TFIIA: characterization of a cDNA encoding the small subunit and requirement for basal and activated transcription. Proc. Nat. Acad. Sci. 92: 3313-3317, 1995. 2. Upadhyaya, A. B., Khan, M., Mou, T.-C., Junker, M., Gray, D. M., DeJong, J. The germ cell-specific transcription factor ALF: structural properties and stabilization of the Tata-binding protein (TBP) -DNA complex. J. Biol. Chem. 277: 34208-34216, 2002. 3. Yudkovsky, N., Ranish, J. A., Hahn, S. A transcription reinitiation intermediate that is stabilized by activator. Nature 408: 225-229, 2000.

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.