Anti-PPAT Antibody Picoband®

The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region.

E.coli-derived human PPAT recombinant protein (Position: D77-Q359) . Human PPAT shares 92.2% and 94.3% amino acid (aa) sequence identity with mouse and rat PPAT, respectively.

1. Brayton, K. A., Chen, Z., Zhou, G., Nagy, P. L., Gavalas, A., Trent, J. M., Deaven, L. L., Dixon, J. E., Zalkin, H. Two genes for de novo purine nucleotide synthesis on human chromosome 4 are closely linked and divergently transcribed. J. Biol. Chem. 269: 5313-5321, 1994. 2. Chu, E. H. Y., Sun, N. C., Chang, C. C. Induction of auxotrophic mutants by treatment of Chinese hamster cells with 5-bromodeoxyuridine and black light. Proc. Nat. Acad. Sci. 69: 3459-3463, 1972. 3. Feldman, R. I., Taylor, M. W. Purine mutants of mammalian cell lines. II. Identification of a phosphoribosylpyrophosphate amidotransferase-deficient mutant of Chinese hamster lung cells. Biochem. Genet. 13: 227-234, 1975.

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.