Human IL-8/Interleukin-8/CXCL8 PicoKine&reg Quick ELISA Kit

Human IL-8/Interleukin-8/CXCL8 PicoKine&reg Quick ELISA Kit (90 minutes, 96 Tests) . Human IL-8/Interleukin-8/CXCL8 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Interleukin-8, also called neutrophil-activating peptide-1 or SCYB8, is a tissue-derived peptide secreted by several types of cells in response to inflammatory stimuli. Monocyte-derived neutrophil chemotactic factor (MDNCF/IL-8, suggested gene symbol IL8) is a cytokine that chemoattracts and activates neutrophils. IL-8 is produced and released from human adipose tissue and from isolated adipocytes in vitro, which may indicate that IL-8 from adipose tissue could be involved in some of the obesity-related complications. The MDNCF/IL-8 gene is placed on the human gene map at position 4q12-q21. This is the same location where at least three other members (platelet factor 4, melanoma growth stimulatory activity, and interferon-gamma induced factor) of the platelet factor 4 gene superfamily reside. Human IL-8 consists of 99 amino acids in precursor form and 79 amino acids in mature form.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 16 16 15 17 16 0.7 4.3% Sample 2 66 67 72 74 69 3.34 4.8% Sample 3 234 249 255 221 239 13.25 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 16 16 15 17 16 0.7 4.3% Sample 1 16 16 15 17 16 0.7 4.3% Sample 2 66 67 72 74 69 3.34 4.8% Sample 2 66 67 72 74 69 3.34 4.8% Sample 3 234 249 255 221 239 13.25 5.5% Sample 3 234 249 255 221 239 13.25 5.5%

Synonyms

Interleukin-8; IL-8; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Emoctakin; Granulocyte chemotactic protein 1; GCP-1; Monocyte-derived neutrophil chemotactic factor; MDNCF; Monocyte-derived neutrophil-activating peptide; MONAP; Neutrophil-activating protein 1; NAP-1; Protein 3-10C; T-cell chemotactic factor; MDNCF-a; GCP/IL-8 protein IV; IL8/NAP1 form I; Interleukin-8; (Ala-IL-8) 77; GCP/IL-8 protein II; IL-8 (1-77) ; IL8/NAP1 form II; MDNCF-b; IL-8 (5-77) ; IL-8 (6-77) ; (Ser-IL-8) 72; GCP/IL-8 protein I; IL8/NAP1 form III; Lymphocyte-derived neutrophil-activating factor; LYNAP; MDNCF-c; Neutrophil-activating factor; NAF; IL-8 (7-77) ; GCP/IL-8 protein V; IL8/NAP1 form IV; IL-8 (8-77) ; GCP/IL-8 protein VI; IL8/NAP1 form V; IL-8 (9-77) ; GCP/IL-8 protein III; IL8/NAP1 form VI; CXCL8; IL8

Gene Name

CXCL8

Gene ID

3576

UniProt

P10145

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S28-S99

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Angiogenesis, Cancer, Cardiovascular, Cytokines, Immunology, Innate Immunity, Interleukins, Invasion/Microenvironment

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 16 66 234 16 63 230 Standard deviation 0.72 2.83 14.27 0.8 3.84 17.48 CV (%) 4.5% 4.3% 6.1% 5% 6.1% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 16 66 234 16 63 230 Standard deviation 0.72 2.83 14.27 0.8 3.84 17.48 CV (%) 4.5% 4.3% 6.1% 5% 6.1% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 16 66 234 16 63 230 Mean (pg/ml) 16 66 234 16 63 230 Standard deviation 0.72 2.83 14.27 0.8 3.84 17.48 Standard deviation 0.72 2.83 14.27 0.8 3.84 17.48 CV (%) 4.5% 4.3% 6.1% 5% 6.1% 7.6% CV (%) 4.5% 4.3% 6.1% 5% 6.1% 7.6%

Sensitivity

<1 pg/mL

Function

IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8 (6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8 (5-77) has increased activity on neutrophil activation and IL-8 (7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8 (1-77), respectively. .

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

11 kDa

Specificity

Natural and recombinant human IL-8

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Interleukin-8

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the intercrine alpha (chemokine CxC) family.

Protein Name

Interleukin-8

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.000 0.082 0.149 0.281 0.556 0.936 1.594 2.071 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.000 0.082 0.149 0.281 0.556 0.936 1.594 2.071 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.000 0.082 0.149 0.281 0.556 0.936 1.594 2.071 O.D. 0.000 0.082 0.149 0.281 0.556 0.936 1.594 2.071

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.