Human IL-7/Interleukin-7 PicoKine&reg Quick ELISA Kit

Human IL-7/Interleukin-7 PicoKine&reg Quick ELISA Kit (90 minutes, 96 Tests) . Human IL-7/Interleukin-7 in cell culture supernatants, serum and plasma (EDTA) . Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

IL-7 a hematopoietic growth factor secreted by stromal cells in the red marrow and thymus. It is also produced by keratinocytes,1 dendritic cells,2 hepatocytes,3 neurons, and endothelial cells4. Interleukin 7 (IL7) is a protein5 that in humans is encoded by the IL7 gene.6, 7, 8Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival.9 IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V (D) J rearrangement of the T cell receptor beta (TCRB) during early T cell development.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 17 18 18 18 17 0.43 2.5% Sample 2 81 89 83 75 82 5 6% Sample 3 209 226 204 221 215 8.86 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 17 18 18 18 17 0.43 2.5% Sample 1 17 18 18 18 17 0.43 2.5% Sample 2 81 89 83 75 82 5 6% Sample 2 81 89 83 75 82 5 6% Sample 3 209 226 204 221 215 8.86 4.1% Sample 3 209 226 204 221 215 8.86 4.1%

Synonyms

Interleukin-7 ; IL-7

Gene Name

IL7

Gene ID

3574

UniProt

P13232

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: D26-H177

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cytokines, Immunology, Innate Immunity, Interleukins

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (EDTA) .

Detection Range

7.8 pg/mL - 500 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 17 81 209 15 78 192 Standard deviation 0.98 5.50 9.82 1.00 5.38 10.56 CV (%) 5.8% 6.9% 4.7% 6.7% 6.9% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 17 81 209 15 78 192 Standard deviation 0.98 5.50 9.82 1.00 5.38 10.56 CV (%) 5.8% 6.9% 4.7% 6.7% 6.9% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 17 81 209 15 78 192 Mean (pg/ml) 17 81 209 15 78 192 Standard deviation 0.98 5.50 9.82 1.00 5.38 10.56 Standard deviation 0.98 5.50 9.82 1.00 5.38 10.56 CV (%) 5.8% 6.9% 4.7% 6.7% 6.9% 5.5% CV (%) 5.8% 6.9% 4.7% 6.7% 6.9% 5.5%

Sensitivity

<1 pg/mL

Function

Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. .

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

20 kDa

Specificity

Natural and recombinant human IL-7

Anticoagulant

EDTA

Gene Name Synonym

Interleukin-7

Subcellular Location

Secreted .

Protein Name

Interleukin-7

Sample Data

Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.013 0.064 0.098 0.196 0.411 0.630 1.137 1.926 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.013 0.064 0.098 0.196 0.411 0.630 1.137 1.926 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.013 0.064 0.098 0.196 0.411 0.630 1.137 1.926 O.D. 0.013 0.064 0.098 0.196 0.411 0.630 1.137 1.926

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.