Human IL-1 Alpha/IL-1F1/IL1A PicoKine&reg Quick ELISA Kit

Human IL-1 Alpha PicoKine&reg Quick ELISA Kit (90 minutes, 96 Tests) . Human IL-1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 0.5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Interleukin-1 alpha (IL-1 alpha) and interleukin-1 beta (IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors. The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin (IL) -1alpha and IL-1beta have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant human IL-1alpha with the molecular mass of 18kDa.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 14 15 15 13 14 0.82 5.8% Sample 2 52 48 50 48 49 1.65 3.3% Sample 3 99 98 112 102 102 5.53 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 14 15 15 13 14 0.82 5.8% Sample 1 14 15 15 13 14 0.82 5.8% Sample 2 52 48 50 48 49 1.65 3.3% Sample 2 52 48 50 48 49 1.65 3.3% Sample 3 99 98 112 102 102 5.53 5.4% Sample 3 99 98 112 102 102 5.53 5.4%

Synonyms

Interleukin-1 alpha; IL-1 alpha; Hematopoietin-1; IL1A; IL1F1

Gene Name

IL1A

Gene ID

3552

UniProt

P01583

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S113-A271

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Tumor Immunology

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

4.7 pg/mL - 300 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 14 52 99 15 49 103 Standard deviation 0.98 2.49 4.35 1.06 2.4 6.07 CV (%) 7% 4.8% 4.4% 7.1% 4.9% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 14 52 99 15 49 103 Standard deviation 0.98 2.49 4.35 1.06 2.4 6.07 CV (%) 7% 4.8% 4.4% 7.1% 4.9% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 14 52 99 15 49 103 Mean (pg/ml) 14 52 99 15 49 103 Standard deviation 0.98 2.49 4.35 1.06 2.4 6.07 Standard deviation 0.98 2.49 4.35 1.06 2.4 6.07 CV (%) 7% 4.8% 4.4% 7.1% 4.9% 5.9% CV (%) 7% 4.8% 4.4% 7.1% 4.9% 5.9%

Sensitivity

<0.5 pg/mL

Function

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

31 kDa

Specificity

Natural and recombinant human IL-1 alpha

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Interleukin-1 alpha

Subcellular Location

Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

Sequence Similarities

Belongs to the IL-1 family.

Protein Name

Interleukin-1 alpha

Sample Data

Concentration (pg/ml) 0 4.7 9.4 18.8 37.5 75 150 300 O.D. 0.041 0.097 0.162 0.367 0.582 0.866 1.335 2.033 Concentration (pg/ml) 0 4.7 9.4 18.8 37.5 75 150 300 O.D. 0.041 0.097 0.162 0.367 0.582 0.866 1.335 2.033 Concentration (pg/ml) 0 4.7 9.4 18.8 37.5 75 150 300 Concentration (pg/ml) 0 4.7 9.4 18.8 37.5 75 150 300 O.D. 0.041 0.097 0.162 0.367 0.582 0.866 1.335 2.033 O.D. 0.041 0.097 0.162 0.367 0.582 0.866 1.335 2.033

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.