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HRP Conjugated AffiniPure Goat Anti-Human IgM

HRP Conjugated Goat Anti-human IgM (u-chain specific) secondary antibody This HRP conjugated antibody is specific for human IgM and shows no cross-reactivity with human IgA/IgG.

Product Specifications

Background

Secondary antibodies offer increased versatility enabling users to use many detection systems such as HRP, AP and fluorescence. They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody, and secondary antibodies' FC regions provide further binding locations for biotin, enable the use of ABC and SABC to further amplify the signal. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. They are then purified through immunoaffinity chromatography and modified with antibody fragmentation, label conjugation, etc., to generate highly specific reagents.

Host

Goat

Reactivity

Human

Immunogen

Human IgM (whole molecule) .

Clonality

Polyclonal

Applications

ELISA, WB

Purification

The antibody was purified from antisera by immunoaffinity chromatography.

Concentration

1mg/ml

Form

Concentrated, Liquid

Storage Conditions

At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Specificity

This HRP conjugated antibody is specific for human IgM and shows no cross-reactivity with human IgA/IgG.

Applications Notes

Western Blotting: 0.1-0.2μg/ml (ECL detection) Western Blotting: 0.7-3.3μg/ml (DAB detection) ELISA: 0.05-0.5μg/ml (TMB detection)

Isotype

IgM

Contents

0.25 mg of HRP conjugated specific antibody, 0.01 M PBS (pH7.4), 50% glycerol.

Conjugate

HRP

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