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Goat Anti-Rabbit IgG (H+L) Secondary Antibody, Fluoro594 Conjugated

This antibody is purified from antiserum by immunoaffinity chromatography which removes essentially all goat serum proteins, except the specific antibody for rabbit IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity in tissue or cell preparations.

Product Specifications

Background

Secondary antibodies offer increased versatility enabling users to use many detection systems such as HRP, AP and fluorescence. They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody, and secondary antibodies' FC regions provide further binding locations for biotin, enable the use of ABC and SABC to further amplify the signal. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. They are then purified through immunoaffinity chromatography and modified with antibody fragmentation, label conjugation, etc., to generate highly specific reagents.

Host

Goat

Reactivity

Rabbit

Immunogen

Rabbit IgG (whole molecule) .

Clonality

Polyclonal

Applications

Flow Cytometry, IF

Concentration

1mg/ml

Storage Conditions

At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Specificity

This Fluoro594 conjugated antibody is specific for rabbit IgG and shows no cross-reactivity with mouse/bovine IgG.

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