Human FGF9 PicoKine ELISA Kit

Fibroblast growth factor-9 (FGF-9) is a steroid-regulated mitogen and survival factor for nerve and mesenchymal cells. The human FGF-9 cDNA cloned by using oligonucleotide probes encodes a polypeptide consisting of 208 amino acids. Sequence similarity to other members of the FGF family has been estimated to be around 30%. FGF-9 is an autocrine estromedin endometrial stromal growth factor that plays roles in cyclic proliferation of uterine endometrial stroma. FGF9 is produced and secreted by the prostatic stromal cells. It is a potent mitogen for both prostatic epithelial and stromal cells in culture. FGF9 is an abundant secreted growth factor that can act as both a paracrine mitogen for epithelial cells and an autocrine mitogen for stromal cells. Overexpression of this paracrine and autocrine growth factor may play an important role in the epithelial and stromal proliferation in benign prostatic hyperplasia.4 As a result of glycosylation, the molecular mass is 25-27KDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 112 101 99 105 104 4.96 4.7% Sample 2 506 485 519 502 503 12.14 2.4% Sample 3 1252 1335 1172 1129 1222 78.76 6.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 112 101 99 105 104 4.96 4.7% Sample 1 112 101 99 105 104 4.96 4.7% Sample 2 506 485 519 502 503 12.14 2.4% Sample 2 506 485 519 502 503 12.14 2.4% Sample 3 1252 1335 1172 1129 1222 78.76 6.4% Sample 3 1252 1335 1172 1129 1222 78.76 6.4%

Elbow knee synostosis; FGF 9; FGF-9; FGF9; FGF9_HUMAN; Fibroblast growth factor 9; GAF; Glia Activating Factor; Glia-activating factor; HBFG 9; HBFG9; HBGF-9; Heparin binding growth factor 9; Heparin-binding growth factor 9; MGC119914; MGC119915; SYNS3

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 112 506 1252 115 527 1200 Standard deviation 6.72 30.86 88.89 7.13 35.83 96 CV (%) 6% 6.1% 7.1% 6.2% 6.8% 8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 112 506 1252 115 527 1200 Standard deviation 6.72 30.86 88.89 7.13 35.83 96 CV (%) 6% 6.1% 7.1% 6.2% 6.8% 8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 112 506 1252 115 527 1200 Mean (pg/ml) 112 506 1252 115 527 1200 Standard deviation 6.72 30.86 88.89 7.13 35.83 96 Standard deviation 6.72 30.86 88.89 7.13 35.83 96 CV (%) 6% 6.1% 7.1% 6.2% 6.8% 8% CV (%) 6% 6.1% 7.1% 6.2% 6.8% 8%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.063 0.113 0.17 0.283 0.485 0.878 1.485 2.13 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.063 0.113 0.17 0.283 0.485 0.878 1.485 2.13 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.063 0.113 0.17 0.283 0.485 0.878 1.485 2.13 O.D. 0.063 0.113 0.17 0.283 0.485 0.878 1.485 2.13

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.