Mouse IgG ELISA Kit PicoKine®

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 2827 2502 1822 1646 2199 166.48 7.6% Sample 2 9440 9016 9079 12073 9902 370.33 3.7% Sample 3 46337 28630 42589 36320 38469 2281.21 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 2827 2502 1822 1646 2199 166.48 7.6% Sample 1 2827 2502 1822 1646 2199 166.48 7.6% Sample 2 9440 9016 9079 12073 9902 370.33 3.7% Sample 2 9440 9016 9079 12073 9902 370.33 3.7% Sample 3 46337 28630 42589 36320 38469 2281.21 5.9% Sample 3 46337 28630 42589 36320 38469 2281.21 5.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2827 9440 46337 3051 7802 41350 Standard deviation 144.46 446.51 2706.08 226.99 183.35 1877.29 CV (%) 5.1% 4.7% 5.8% 7.4% 2.4% 4.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2827 9440 46337 3051 7802 41350 Standard deviation 144.46 446.51 2706.08 226.99 183.35 1877.29 CV (%) 5.1% 4.7% 5.8% 7.4% 2.4% 4.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 2827 9440 46337 3051 7802 41350 Mean (pg/ml) 2827 9440 46337 3051 7802 41350 Standard deviation 144.46 446.51 2706.08 226.99 183.35 1877.29 Standard deviation 144.46 446.51 2706.08 226.99 183.35 1877.29 CV (%) 5.1% 4.7% 5.8% 7.4% 2.4% 4.5% CV (%) 5.1% 4.7% 5.8% 7.4% 2.4% 4.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.001 0.121 0.21 0.375 0.684 1.145 1.628 2.038 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.001 0.121 0.21 0.375 0.684 1.145 1.628 2.038 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.001 0.121 0.21 0.375 0.684 1.145 1.628 2.038 O.D. 0.001 0.121 0.21 0.375 0.684 1.145 1.628 2.038

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.