Mouse TNF Beta ELISA Kit PicoKine®

The encoded protein, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4, myocardial infarction, non-Hodgkin's lymphoma, and psoriatic arthritis. Alternatively spliced transcript variants have been observed for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 244 208 192 307 238 8.92 3.8% Sample 2 898 818 924 824 866 56.20 6.5% Sample 3 4739 3374 2777 4604 3874 98.00 2.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 244 208 192 307 238 8.92 3.8% Sample 1 244 208 192 307 238 8.92 3.8% Sample 2 898 818 924 824 866 56.20 6.5% Sample 2 898 818 924 824 866 56.20 6.5% Sample 3 4739 3374 2777 4604 3874 98.00 2.5% Sample 3 4739 3374 2777 4604 3874 98.00 2.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 244 898 4739 158 1201 3122 Standard deviation 13.91/td> 19.40 356.85 6.23 49.36 105.52 CV (%) 5.7% 2.2%% 7.5% 3.9% 4.1% 3.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 244 898 4739 158 1201 3122 Standard deviation 13.91/td> 19.40 356.85 6.23 49.36 105.52 CV (%) 5.7% 2.2%% 7.5% 3.9% 4.1% 3.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 244 898 4739 158 1201 3122 Mean (pg/ml) 244 898 4739 158 1201 3122 Standard deviation 13.91/td> 19.40 356.85 6.23 49.36 105.52 Standard deviation 13.91/td> 19.40 356.85 6.23 49.36 105.52 CV (%) 5.7% 2.2%% 7.5% 3.9% 4.1% 3.4% CV (%) 5.7% 2.2%% 7.5% 3.9% 4.1% 3.4%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.053 0.112 0.156 0.227 0.374 0.813 1.47 2.373 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.053 0.112 0.156 0.227 0.374 0.813 1.47 2.373 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.053 0.112 0.156 0.227 0.374 0.813 1.47 2.373 O.D. 0.053 0.112 0.156 0.227 0.374 0.813 1.47 2.373

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.