Human IL-33 PicoKine® Quick ELISA Kit

Human IL-33 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Human IL33 in cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Interleukin 33 (IL-33) is a cytokine belonging to the IL-1 superfamily. The IL33 gene maps to chromosome 9p24.1 by using of genomic database analysis. Recombinant mature human IL33 bound to ST2. The induction of type 2 cytokines by IL-33 in vivo is believed to induce the severe pathological changes observed in mucosal organs following administration of IL-33. IL33, an alarmin released from necrotic cells, is necessary for potent CD8 + T cell (CTL) responses to replicating, prototypic RNA, and DNA viruses in mice. IL33 prevented the downregulation of CXCR2 and inhibition of chemotaxis induced by activation of TLR4, and found that IL33 reverses the TLR4-induced reduction of CXCR2 expression via the inhibition of expression of GRK2.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 30 31 28 32 30 1.47 4.9% Sample 2 260 230 231 235 239 12.26 5.1% Sample 3 484 481 517 495 494 14.13 2.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 30 31 28 32 30 1.47 4.9% Sample 1 30 31 28 32 30 1.47 4.9% Sample 2 260 230 231 235 239 12.26 5.1% Sample 2 260 230 231 235 239 12.26 5.1% Sample 3 484 481 517 495 494 14.13 2.8% Sample 3 484 481 517 495 494 14.13 2.8%

Synonyms

Interleukin-33; IL-33; Interleukin-1 family member 11; IL-1F11; Nuclear factor from high endothelial venules; NF-HEV; Interleukin-33 (95-270) ; Interleukin-33 (99-270) ; Interleukin-33 (109-270) ; IL33; C9orf26, IL1F11, NFHEV

Gene Name

IL33

Gene ID

90865

UniProt

O95760

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S112-T270

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta. .

Applications

ELISA

Field of Research

Angiogenesis, Cardiovascular, Cytokines, Hypertrophy, Immunology, Innate Immunity, Interleukins

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates.

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 30 260 484 28 282 511 Standard deviation 1.74 17.98 37.75 1.76 22.27 47.89 CV (%) 5.8% 7.9% 7.8% 7.9% 7.9% 9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 30 260 484 28 282 511 Standard deviation 1.74 17.98 37.75 1.76 22.27 47.89 CV (%) 5.8% 7.9% 7.8% 7.9% 7.9% 9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 30 260 484 28 282 511 Mean (pg/ml) 30 260 484 28 282 511 Standard deviation 1.74 17.98 37.75 1.76 22.27 47.89 Standard deviation 1.74 17.98 37.75 1.76 22.27 47.89 CV (%) 5.8% 7.9% 7.8% 7.9% 7.9% 9% CV (%) 5.8% 7.9% 7.8% 7.9% 7.9% 9%

Sensitivity

<10 pg/mL

Function

Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:16286016) . Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an alarmin, that amplifies immune responses during tissue injury (PubMed:17853410, PubMed:18836528) . .

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

31 kDa

Specificity

Natural and recombinant human IL-33

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin-33

Subcellular Location

Nucleus. Chromosome. Cytoplasmic vesicle, secretory vesicle. Secreted. Associates with heterochromatin and mitotic chromosomes. Translocation from the nucleus occurs upon biomechanical strain, depends on an intact microtubule network, and is ATP-dependent.

Protein Name

Interleukin-33

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.011 0.103 0.168 0.312 0.609 0.933 1.546 2.068 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.011 0.103 0.168 0.312 0.609 0.933 1.546 2.068 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.011 0.103 0.168 0.312 0.609 0.933 1.546 2.068 O.D. 0.011 0.103 0.168 0.312 0.609 0.933 1.546 2.068

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.