Mouse MPO/Myeloperoxidase PicoKine® Quick ELISA Kit

Mouse MPO/Myeloperoxidase PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Mouse Mpo in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Myeloperoxidase (MPO) is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 354 346 367 309 344 21.55 6.2% Sample 2 1809 1947 1689 1851 1824 92.61 5% Sample 3 5328 4954 4733 4680 4923 255.01 5.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 354 346 367 309 344 21.55 6.2% Sample 1 354 346 367 309 344 21.55 6.2% Sample 2 1809 1947 1689 1851 1824 92.61 5% Sample 2 1809 1947 1689 1851 1824 92.61 5% Sample 3 5328 4954 4733 4680 4923 255.01 5.1% Sample 3 5328 4954 4733 4680 4923 255.01 5.1%

Synonyms

Myeloperoxidase; MPO;1.11.2.2; Myeloperoxidase light chain; Myeloperoxidase heavy chain; Mpo

Gene Name

MPO

Gene ID

17523

UniProt

P11247

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: M16-T718

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cancer, Cancer Metabolism, Cardiovascular, Immunology, Innate Immunity, Macrophage/Inflammation, Metabolism, Pathways and Processes, Redox Metabolism, Vasculature

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 354 1809 5328 362 1718 5171 Standard deviation 19.11 115.77 266.4 346.457 137.44 346.45 CV (%) 5.4% 6.4% 5% 6.9% 8% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 354 1809 5328 362 1718 5171 Standard deviation 19.11 115.77 266.4 346.457 137.44 346.45 CV (%) 5.4% 6.4% 5% 6.9% 8% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 354 1809 5328 362 1718 5171 Mean (pg/ml) 354 1809 5328 362 1718 5171 Standard deviation 19.11 115.77 266.4 346.457 137.44 346.45 Standard deviation 19.11 115.77 266.4 346.457 137.44 346.45 CV (%) 5.4% 6.4% 5% 6.9% 8% 6.7% CV (%) 5.4% 6.4% 5% 6.9% 8% 6.7%

Sensitivity

<10 pg/mL

Function

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (By similarity) . .

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

81 kDa

Specificity

Natural and recombinant mouse MPO

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Myeloperoxidase

Subcellular Location

Lysosome.

Protein Name

Myeloperoxidase

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.034 0.070 0.108 0.184 0.427 0.660 1.378 2.095 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.034 0.070 0.108 0.184 0.427 0.660 1.378 2.095 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.034 0.070 0.108 0.184 0.427 0.660 1.378 2.095 O.D. 0.034 0.070 0.108 0.184 0.427 0.660 1.378 2.095

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.