Human Fibronectin PicoKine® Quick ELISA Kit

Fibronectin (FN) also known as LETS, is identified on the surfFN of fibroblasts by labeling with radioactive compounds or specific antibodies. Fibronectin is a 430,000-dalton dimeric glycoprotein that exists in 2 forms, termed cellular and plasma fibronectin. Cellular and plasma fibronectins are heterodimers consisting of similar but not identical polypeptides. These two forms of FN differ in biologic activity. Fibronectins bind cell surfFNs and various compounds including collagen, fibrin, heparin, DNA, and actin. Because fibronectin stimulates endocytosis in several systems and promotes the clearance of particulate material from the circulation, it could function in the clearance of C1q-coated material such as immune complexes or cellular debris. Fibronectins are involved in cell adhesion, cell motility, opsonization, would healing, and maintenance of cell shape. LETS, encoded on chromosome 8, is responsible for the LETS protein expression in humans. Because LETS has been implicated in tumorigenicity and cellular transformation, it is of interest that rearrangement or modifications in the number of chromosome 8 have been associated with certain forms of cancer. The standard used in this kit is isolated from human plasma with the molecular mass of 200-250KDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 312 333 321 275 310 21.67 6.9% Sample 2 1118 1155 1256 1278 1201 67 5.5% Sample 3 5412 5418 5472 5364 5416 38.27 0.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 312 333 321 275 310 21.67 6.9% Sample 1 312 333 321 275 310 21.67 6.9% Sample 2 1118 1155 1256 1278 1201 67 5.5% Sample 2 1118 1155 1256 1278 1201 67 5.5% Sample 3 5412 5418 5472 5364 5416 38.27 0.7% Sample 3 5412 5418 5472 5364 5416 38.27 0.7%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 312 1118 5412 341 1105 4997 Standard deviation 14.35 65.76 308.48 19.43 76.24 349.79 CV (%) 4.6% 5.7% 5.7% 5.7% 6.9% 7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 312 1118 5412 341 1105 4997 Standard deviation 14.35 65.76 308.48 19.43 76.24 349.79 CV (%) 4.6% 5.7% 5.7% 5.7% 6.9% 7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 312 1118 5412 341 1105 4997 Mean (pg/ml) 312 1118 5412 341 1105 4997 Standard deviation 14.35 65.76 308.48 19.43 76.24 349.79 Standard deviation 14.35 65.76 308.48 19.43 76.24 349.79 CV (%) 4.6% 5.7% 5.7% 5.7% 6.9% 7% CV (%) 4.6% 5.7% 5.7% 5.7% 6.9% 7%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.052 0.091 0.186 0.317 0.490 0.890 1.385 1.990 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.052 0.091 0.186 0.317 0.490 0.890 1.385 1.990 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.052 0.091 0.186 0.317 0.490 0.890 1.385 1.990 O.D. 0.052 0.091 0.186 0.317 0.490 0.890 1.385 1.990

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.