Human HGF/Hepatocyte growth factor PicoKine® Quick ELISA Kit

Human HGF/Hepatocyte growth factor PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human HGF in cell culture supernatants, serum and plasma (EDTA, citrate) . Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Hepatocyte growth factor (HGF) is the most potent mitogen for mature parenchymal hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. HGF has a relative molecular mass (Mr) of 82,000 and is a heterodimer composed of a large alpha-subunit of Mr 69,000 and a small beta-subunit of Mr 34,000. The protein consists of 728 amino acid residues, including a possible signal peptide at the N-terminus. HGF may serve as a paracrine mediator to control placental development and growth. This growth factor may play an important role as a paracrine mediator of the proliferation of melanocytes and endothelial cells, as well as cells of epithelial origin. The gene encoding the human HGF is assigned to human chromosome 7.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 169 159 165 152 161 6.41 3.9% Sample 2 1246 1103 1132 1240 1180 63.61 5.3% Sample 3 4183 3905 4308 3657 4013 252.15 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 169 159 165 152 161 6.41 3.9% Sample 1 169 159 165 152 161 6.41 3.9% Sample 2 1246 1103 1132 1240 1180 63.61 5.3% Sample 2 1246 1103 1132 1240 1180 63.61 5.3% Sample 3 4183 3905 4308 3657 4013 252.15 6.2% Sample 3 4183 3905 4308 3657 4013 252.15 6.2%

Synonyms

Hepatocyte growth factor; Hepatopoietin-A; Scatter factor; SF; Hepatocyte growth factor alpha chain; Hepatocyte growth factor beta chain; HGF; HPTA

Gene Name

HGF

Gene ID

3082

UniProt

P14210

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q32-R494 (alpha) & V495-S728 (beta)

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (EDTA, citrate) .

Detection Range

125 pg/mL - 8,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 169 1246 4183 168 1364 4239 Standard deviation 9.8 87.22 267.71 9.91 118.66 313.68 CV (%) 5.8% 7% 6.4% 5.9% 8.7% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 169 1246 4183 168 1364 4239 Standard deviation 9.8 87.22 267.71 9.91 118.66 313.68 CV (%) 5.8% 7% 6.4% 5.9% 8.7% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 169 1246 4183 168 1364 4239 Mean (pg/ml) 169 1246 4183 168 1364 4239 Standard deviation 9.8 87.22 267.71 9.91 118.66 313.68 Standard deviation 9.8 87.22 267.71 9.91 118.66 313.68 CV (%) 5.8% 7% 6.4% 5.9% 8.7% 7.4% CV (%) 5.8% 7% 6.4% 5.9% 8.7% 7.4%

Sensitivity

<5 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

83 kDa

Specificity

Natural and recombinant human HGF

Anticoagulant

EDTA or citrate

Gene Name Synonym

Hepatocyte growth factor

Protein Name

Hepatocyte growth factor

Sample Data

Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.014 0.081 0.116 0.217 0.357 0.631 1.261 2.033 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.014 0.081 0.116 0.217 0.357 0.631 1.261 2.033 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.014 0.081 0.116 0.217 0.357 0.631 1.261 2.033 O.D. 0.014 0.081 0.116 0.217 0.357 0.631 1.261 2.033

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.