Human CHRDL1 PicoKine® Quick ELISA Kit

Human CHRDL1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human CHRDL1 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chordin-like 1 is a protein that in humans is encoded by the CHRDL1 gene. It is mapped to Xq23. This gene encodes an antagonist of bone morphogenetic protein 4. The encoded protein may play a role in topographic retinotectal projection and in the regulation of retinal angiogenesis in response to hypoxia. Alternatively spliced transcript variants encoding different isoforms have been described.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 146 138 126 133 135 7.29 5.4% Sample 2 511 424 442 453 457 32.57 7.1% Sample 3 1796 1945 1901 2041 1920 88.03 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 146 138 126 133 135 7.29 5.4% Sample 1 146 138 126 133 135 7.29 5.4% Sample 2 511 424 442 453 457 32.57 7.1% Sample 2 511 424 442 453 457 32.57 7.1% Sample 3 1796 1945 1901 2041 1920 88.03 4.5% Sample 3 1796 1945 1901 2041 1920 88.03 4.5%

Synonyms

Chordin-like protein 1; Neuralin-1; Neurogenesin-1; Ventroptin; CHRDL1; NRLN1

Gene Name

CHRDL1

Gene ID

91851

UniProt

Q9BU40

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E22-C450

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

93.6 pg/mL - 6,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 146 511 1796 201 618 2851 Standard deviation 7.59 27.08 100.58 13.07 41.41 196.72 CV (%) 5.2% 5.3% 5.6% 6.5% 6.7% 6.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 146 511 1796 201 618 2851 Standard deviation 7.59 27.08 100.58 13.07 41.41 196.72 CV (%) 5.2% 5.3% 5.6% 6.5% 6.7% 6.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 146 511 1796 201 618 2851 Mean (pg/ml) 146 511 1796 201 618 2851 Standard deviation 7.59 27.08 100.58 13.07 41.41 196.72 Standard deviation 7.59 27.08 100.58 13.07 41.41 196.72 CV (%) 5.2% 5.3% 5.6% 6.5% 6.7% 6.9% CV (%) 5.2% 5.3% 5.6% 6.5% 6.7% 6.9%

Sensitivity

<50 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

52 KDa

Specificity

Natural and recombinant human CHRDL1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Chordin like 1

Protein Name

Chordin like 1

Sample Data

Concentration (pg/ml) 0 93.7 187.5 375 750 1500 3000 6000 O.D. 0.088 0.134 0.176 0.265 0.402 0.702 1.263 2.099 Concentration (pg/ml) 0 93.7 187.5 375 750 1500 3000 6000 O.D. 0.088 0.134 0.176 0.265 0.402 0.702 1.263 2.099 Concentration (pg/ml) 0 93.7 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.7 187.5 375 750 1500 3000 6000 O.D. 0.088 0.134 0.176 0.265 0.402 0.702 1.263 2.099 O.D. 0.088 0.134 0.176 0.265 0.402 0.702 1.263 2.099

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.