Human AFP/Alpha-fetoprotein PicoKine® Quick ELISA Kit

Human AFP/Alpha-fetoprotein PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human AFP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Alpha-fetoprotein (AFP, α-fetoprotein; also sometimes called alpha-1-fetoprotein, alpha-fetoglobulin, or alpha fetal protein) is a protein that in humans is encoded by the AFP gene. The AFP gene is located on the q arm of chromosome 4 (4q25) . AFP is the most abundant plasma protein found in the human fetus. Plasma levels decrease rapidly after birth but begin decreasing prenatally starting at the end of the first trimester. Normal adult levels are usually achieved by the age of 8 to 12 months. The function of AFP in adult humans is unknown; however, in rodents it binds estradiol to prevent the transport of this hormone across the placenta to the fetus. The main function of this is to prevent the virilization of female fetuses. As human AFP does not bind estrogen, its function in humans is less clear.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 95 105 109 107 104 5.38 5.1% Sample 2 643 732 682 710 691 33.25 4.8% Sample 3 2190 2290 2420 2404 2326 93.15 4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 95 105 109 107 104 5.38 5.1% Sample 1 95 105 109 107 104 5.38 5.1% Sample 2 643 732 682 710 691 33.25 4.8% Sample 2 643 732 682 710 691 33.25 4.8% Sample 3 2190 2290 2420 2404 2326 93.15 4% Sample 3 2190 2290 2420 2404 2326 93.15 4%

Synonyms

Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin; AFP; HPAFP

Gene Name

AFP

Gene ID

174

UniProt

P02771

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: R19-V609

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

5 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

78 pg/mL - 5,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 98 643 2190 98 654 1986 Standard deviation 4.56 47.58 100.74 5.58 56.24 129.09 CV (%) 4.8% 7.4% 4.6% 5.7% 8.6% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 98 643 2190 98 654 1986 Standard deviation 4.56 47.58 100.74 5.58 56.24 129.09 CV (%) 4.8% 7.4% 4.6% 5.7% 8.6% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 98 643 2190 98 654 1986 Mean (pg/ml) 98 643 2190 98 654 1986 Standard deviation 4.56 47.58 100.74 5.58 56.24 129.09 Standard deviation 4.56 47.58 100.74 5.58 56.24 129.09 CV (%) 4.8% 7.4% 4.6% 5.7% 8.6% 6.5% CV (%) 4.8% 7.4% 4.6% 5.7% 8.6% 6.5%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 HRP-Linked Antibody|1|6ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

69 kDa

Specificity

Natural and recombinant human AFP

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Alpha-fetoprotein

Protein Name

Alpha-fetoprotein

Sample Data

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.003 0.137 0.248 0.486 0.777 1.260 1.704 1.925 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.003 0.137 0.248 0.486 0.777 1.260 1.704 1.925 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.003 0.137 0.248 0.486 0.777 1.260 1.704 1.925 O.D. 0.003 0.137 0.248 0.486 0.777 1.260 1.704 1.925

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.