Mouse ADAMTS1 PicoKine® Quick ELISA Kit

Mouse ADAMTS1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Mouse Adamts1 in cell culture supernatants, serum and plasma (heparin) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

ADAM metallopeptidase with thrombospondin type 1 motif 1.This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene contains two disintegrin loops and three C-terminal TS motifs and has anti-angiogenic activity. The expression of this gene may be associated with various inflammatory processes as well as development of cancer cachexia. This gene is likely to be necessary for normal growth, fertility, and organ morphology and function.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 99 121 177 174 143 9.85 6.9% Sample 2 409 642 650 377 520 16.31 3.1% Sample 3 1816 2943 2064 2404 2307 179.23 7.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 99 121 177 174 143 9.85 6.9% Sample 1 99 121 177 174 143 9.85 6.9% Sample 2 409 642 650 377 520 16.31 3.1% Sample 2 409 642 650 377 520 16.31 3.1% Sample 3 1816 2943 2064 2404 2307 179.23 7.8% Sample 3 1816 2943 2064 2404 2307 179.23 7.8%

Synonyms

Adamts1; A disintegrin and metalloproteinase with thrombospondin motifs 1; ADAM-TS 1; ADAM-TS1; ADAMTS-1; EC 3.4.24.-

Gene Name

ADAMTS1

Gene ID

11504

UniProt

P97857

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: F254-C725

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin) .

Detection Range

93.8 pg/mL - 6,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 99 409 1816 147 471 1786 Standard deviation 7.9 13.78 140.74 7.2 22.09 140.92 CV (%) 8.0% 3.4% 7.8% 4.9% 4.7% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 99 409 1816 147 471 1786 Standard deviation 7.9 13.78 140.74 7.2 22.09 140.92 CV (%) 8.0% 3.4% 7.8% 4.9% 4.7% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 99 409 1816 147 471 1786 Mean (pg/ml) 99 409 1816 147 471 1786 Standard deviation 7.9 13.78 140.74 7.2 22.09 140.92 Standard deviation 7.9 13.78 140.74 7.2 22.09 140.92 CV (%) 8.0% 3.4% 7.8% 4.9% 4.7% 7.9% CV (%) 8.0% 3.4% 7.8% 4.9% 4.7% 7.9%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

106 kDa

Specificity

Natural and recombinant mouse ADAMTS1

Anticoagulant

Heparin

Gene Name Synonym

ADAM metallopeptidase with thrombospondin type 1 motif 1

Protein Name

A disintegrin and metalloproteinase with thrombospondin motifs 1

Sample Data

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.018 0.080 0.168 0.296 0.602 1.001 1.555 2.000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.018 0.080 0.168 0.296 0.602 1.001 1.555 2.000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.018 0.080 0.168 0.296 0.602 1.001 1.555 2.000 O.D. 0.018 0.080 0.168 0.296 0.602 1.001 1.555 2.000

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.