Mouse PTX3/Pentraxin 3 PicoKine® Quick ELISA Kit

Mouse PTX3/Pentraxin 3 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Mouse Ptx3 in cell culture supernatants, serum and plasma (EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

PTX3 (Pentraxin 3) is a member of the pentraxin superfamily. This super family characterized by cyclic multimeric structure. The PTX3 gene is mapped to 3q25.32. The predicted 381-amino acid PTX3 protein has homology to the pentraxin protein family. Significant levels of PTX3 were detected in plasma of neutropenic patients with systemic A. PTX3 is effective in preventing CMV infection and reactivation, as well as subsequent Aspergillus infection. PTX3 activates the classical pathway of complementactivation and facilitates pathogen recognition by macrophages and DCs.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 262 259 249 272 260 8.2 3.1% Sample 2 1334 1350 1444 1511 1409 71.99 5.1% Sample 3 4146 4596 4000 4523 4316 250 5.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 262 259 249 272 260 8.2 3.1% Sample 1 262 259 249 272 260 8.2 3.1% Sample 2 1334 1350 1444 1511 1409 71.99 5.1% Sample 2 1334 1350 1444 1511 1409 71.99 5.1% Sample 3 4146 4596 4000 4523 4316 250 5.7% Sample 3 4146 4596 4000 4523 4316 250 5.7%

Synonyms

Pentraxin-related protein PTX3; Pentaxin-related protein PTX3; Tumor necrosis factor-inducible gene 14 protein; TSG-14; Ptx3; Tsg14

Gene Name

PTX3

Gene ID

19288

UniProt

P48759

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E18-S381

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 262 1334 4146 264 1451 3917 Standard deviation 15.19 98.71 211.44 15.84 113.17 274.19 CV (%) 5.8% 7.4% 7% 6% 7.8% 7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 262 1334 4146 264 1451 3917 Standard deviation 15.19 98.71 211.44 15.84 113.17 274.19 CV (%) 5.8% 7.4% 7% 6% 7.8% 7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 262 1334 4146 264 1451 3917 Mean (pg/ml) 262 1334 4146 264 1451 3917 Standard deviation 15.19 98.71 211.44 15.84 113.17 274.19 Standard deviation 15.19 98.71 211.44 15.84 113.17 274.19 CV (%) 5.8% 7.4% 7% 6% 7.8% 7% CV (%) 5.8% 7.4% 7% 6% 7.8% 7%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 HRP-Linked Antibody|1|6ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

42 kDa

Specificity

Natural and recombinant mouse PTX3

Anticoagulant

EDTA

Gene Name Synonym

Pentraxin-related protein PTX3

Protein Name

Pentraxin-related protein PTX3

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.035 0.119 0.209 0.344 0.626 1.028 1.542 2.207 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.035 0.119 0.209 0.344 0.626 1.028 1.542 2.207 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.035 0.119 0.209 0.344 0.626 1.028 1.542 2.207 O.D. 0.035 0.119 0.209 0.344 0.626 1.028 1.542 2.207

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.