Mouse IL-1 Beta/IL-1F2/IL1B PicoKine® Quick ELISA Kit

Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone1. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB) -regulated genes2. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells3. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture4. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 34 31 32 32 1.11 3.4% Sample 2 211 206 203 208 207 2.91 1.4% Sample 3 483 452 433 430 449 21.1 4.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 34 31 32 32 1.11 3.4% Sample 1 33 34 31 32 32 1.11 3.4% Sample 2 211 206 203 208 207 2.91 1.4% Sample 2 211 206 203 208 207 2.91 1.4% Sample 3 483 452 433 430 449 21.1 4.6% Sample 3 483 452 433 430 449 21.1 4.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 211 483 36 217 481 Standard deviation 1.74 10.33 34.29 2.19 14.75 35.59 CV (%) 5.3% 4.9% 7.1% 6.1% 6.8% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 211 483 36 217 481 Standard deviation 1.74 10.33 34.29 2.19 14.75 35.59 CV (%) 5.3% 4.9% 7.1% 6.1% 6.8% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 33 211 483 36 217 481 Mean (pg/ml) 33 211 483 36 217 481 Standard deviation 1.74 10.33 34.29 2.19 14.75 35.59 Standard deviation 1.74 10.33 34.29 2.19 14.75 35.59 CV (%) 5.3% 4.9% 7.1% 6.1% 6.8% 7.4% CV (%) 5.3% 4.9% 7.1% 6.1% 6.8% 7.4%

Description|Quantity|Volume Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 HRP-Linked Antibody|1|6ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 12.5 25 50 100 200 400 800 O.D. 0.030 0.065 0.123 0.212 0.336 0.609 1.031 1.898 Concentration (pg/ml) 0 12.5 25 50 100 200 400 800 O.D. 0.030 0.065 0.123 0.212 0.336 0.609 1.031 1.898 Concentration (pg/ml) 0 12.5 25 50 100 200 400 800 Concentration (pg/ml) 0 12.5 25 50 100 200 400 800 O.D. 0.030 0.065 0.123 0.212 0.336 0.609 1.031 1.898 O.D. 0.030 0.065 0.123 0.212 0.336 0.609 1.031 1.898

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.