Human ANGPTL7 PicoKine® Quick ELISA Kit

Human ANGPTL7 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human ANGPTL7 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Angiopoietin-related protein 7 is a protein that in humans is encoded by the ANGPTL7 gene. This gene is mapped to 1p36.22. Angiopoietin-like Protein 7 (ANGPTL7), also known as Corneal-Derived Transcript 6 (CDT6), is a secreted, 45?50 kDa glycoprotein member of the angiopoietin?like family of molecules. Members of this protein family contain an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain. ANGPTL7 is expressed in the corneal stroma, trabecular meshwork, and sclera. Its production is up?regulated in trabecular meshwork cells by glucocorticoids and TGF-beta and in cartilage by TNF-alpha. ANGPTL7 expression is up?regulated under conditions of increased ocular pressure.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 140 129 118 129 129 7.77 6.0% Sample 2 644 660 702 583 647 42.71 6.6% Sample 3 1907 2152 2078 2215 2088 115.20 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 140 129 118 129 129 7.77 6.0% Sample 1 140 129 118 129 129 7.77 6.0% Sample 2 644 660 702 583 647 42.71 6.6% Sample 2 644 660 702 583 647 42.71 6.6% Sample 3 1907 2152 2078 2215 2088 115.20 5.5% Sample 3 1907 2152 2078 2215 2088 115.20 5.5%

Synonyms

Angiopoietin-related protein 7; Angiopoietin-like factor; Angiopoietin-like protein 7; Cornea-derived transcript 6 protein; ANGPTL7; CDT6; UNQ313; PRO356

Gene Name

ANGPTL7

Gene ID

10218

UniProt

O43827

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: Q27-P346

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

78 pg/mL - 5,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 140 644 1907 145 639 2253 Standard deviation 6.86 27.69 97.26 9.57 35.15 173.48 CV (%) 4.9% 4.3% 5.1% 6.6% 5.5% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 140 644 1907 145 639 2253 Standard deviation 6.86 27.69 97.26 9.57 35.15 173.48 CV (%) 4.9% 4.3% 5.1% 6.6% 5.5% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 140 644 1907 145 639 2253 Mean (pg/ml) 140 644 1907 145 639 2253 Standard deviation 6.86 27.69 97.26 9.57 35.15 173.48 Standard deviation 6.86 27.69 97.26 9.57 35.15 173.48 CV (%) 4.9% 4.3% 5.1% 6.6% 5.5% 7.7% CV (%) 4.9% 4.3% 5.1% 6.6% 5.5% 7.7%

Sensitivity

<50 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant human ANGPTL7

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Angiopoietin like 7

Protein Name

Angiopoietin-related protein 7

Sample Data

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.044 0.109 0.162 0.268 0.480 0.828 1.459 2.048 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.044 0.109 0.162 0.268 0.480 0.828 1.459 2.048 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.044 0.109 0.162 0.268 0.480 0.828 1.459 2.048 O.D. 0.044 0.109 0.162 0.268 0.480 0.828 1.459 2.048

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.