Rat IL-1 Beta/IL-1F2/IL1B PicoKine® Quick ELISA Kit

Rat IL-1 Beta/IL-1F2/IL1B PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Rat Il1b in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

The Interleukin 1 family is a group of 11 beta trefoil cytokines. Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response . Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta belongs to proinflammatory cytokines, promote cancer cell adhesion and liver metastases by up-regulating the expression of vascular cell adhesion molecule-1 (VCAM-1) on hepatic sinusoidal endothelium (HSE) .

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 58 59 57 56 57 1.11 1.9% Sample 2 233 268 237 251 247 13.71 5.5% Sample 3 962 826 929 964 920 56.16 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 58 59 57 56 57 1.11 1.9% Sample 1 58 59 57 56 57 1.11 1.9% Sample 2 233 268 237 251 247 13.71 5.5% Sample 2 233 268 237 251 247 13.71 5.5% Sample 3 962 826 929 964 920 56.16 6.1% Sample 3 962 826 929 964 920 56.16 6.1%

Synonyms

Interleukin 1 beta ; Interleukin-1 beta ; Il1b ; rCG_27107

Gene Name

IL1B

Gene ID

24494

UniProt

Q63264

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V117-S268

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 58 233 962 62 241 881 Standard deviation 3.65 11.41 69.26 4.21 12.29 68.71 CV (%) 7.8% 4.9% 7.2% 6.8% 7.8% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 58 233 962 62 241 881 Standard deviation 3.65 11.41 69.26 4.21 12.29 68.71 CV (%) 7.8% 4.9% 7.2% 6.8% 7.8% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 58 233 962 62 241 881 Mean (pg/ml) 58 233 962 62 241 881 Standard deviation 3.65 11.41 69.26 4.21 12.29 68.71 Standard deviation 3.65 11.41 69.26 4.21 12.29 68.71 CV (%) 7.8% 4.9% 7.2% 6.8% 7.8% 7.8% CV (%) 7.8% 4.9% 7.2% 6.8% 7.8% 7.8%

Sensitivity

<1 pg/mL

Components

Description|Quantity|Volume Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 HRP-Linked Antibody|1|6ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

30659 MW

Specificity

Natural and recombinant rat IL-1 beta

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin 1, beta

Protein Name

Interleukin-1 beta

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.031 0.079 0.146 0.207 0.338 0.569 1.051 1.829 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.031 0.079 0.146 0.207 0.338 0.569 1.051 1.829 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.031 0.079 0.146 0.207 0.338 0.569 1.051 1.829 O.D. 0.031 0.079 0.146 0.207 0.338 0.569 1.051 1.829

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.