Mouse MCP-1 PicoKine® Quick ELISA Kit

Mouse MCP-1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Mouse Ccl2 in cell culture supernatants and serum. Sensitivity: 6pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Monocyte chemotactic protein-1 (MCP-1) is a member of the small inducible gene (SIG) family. It has been shown to play a role in the recruitment of monocytes to sites of injury and infection. By analysis of a panel of somatic cell hybrids, we have localized the MCP-1 gene, designated SCYA2, to human chromosome 17. In situ hybridization confirmed this assignment and further localized the gene to 17q11.2-q21.1. MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder. Monocyte chemoattractant protein-1 (MCP-1), regulated on activation, normal T cell expressed and secreted, and stimulation of monocytes from healthy carriers of the genotype GG with Mycobacterium tuberculosis antigens yielded higher MCP-1. The standard used in this kit is recombinant mouse MCP-1 (Q24-R96) with the molecular mass of 8.5Kda.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 39 36 34 34 35 2.04 5.8% Sample 2 224 228 228 219 224 3.69 1.6% Sample 3 394 375 431 394 398 20.3 5.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 39 36 34 34 35 2.04 5.8% Sample 1 39 36 34 34 35 2.04 5.8% Sample 2 224 228 228 219 224 3.69 1.6% Sample 2 224 228 228 219 224 3.69 1.6% Sample 3 394 375 431 394 398 20.3 5.1% Sample 3 394 375 431 394 398 20.3 5.1%

Synonyms

C-C motif chemokine 2; Monocyte chemoattractant protein 1; Monocyte chemotactic protein 1; MCP-1; Platelet-derived growth factor-inducible protein JE; Small-inducible cytokine A2; Ccl2; Je, Mcp1, Scya2

Gene Name

CCL2

Gene ID

20296

UniProt

P10148

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Q24-R96

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants and serum.

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 39 224 394 40 231 398 Standard deviation 1.56 10.75 27.18 2 12.47 27.46 CV (%) 4% 4.8% 6.9% 5% 5.4% 6.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 39 224 394 40 231 398 Standard deviation 1.56 10.75 27.18 2 12.47 27.46 CV (%) 4% 4.8% 6.9% 5% 5.4% 6.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 39 224 394 40 231 398 Mean (pg/ml) 39 224 394 40 231 398 Standard deviation 1.56 10.75 27.18 2 12.47 27.46 Standard deviation 1.56 10.75 27.18 2 12.47 27.46 CV (%) 4% 4.8% 6.9% 5% 5.4% 6.9% CV (%) 4% 4.8% 6.9% 5% 5.4% 6.9%

Sensitivity

<6 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

16326 MW

Specificity

Natural and recombinant mouse MCP-1

Gene Name Synonym

C-C motif chemokine 2

Protein Name

C-C motif chemokine 2

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.014 0.051 0.090 0.193 0.406 0.726 1.465 1.897 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.014 0.051 0.090 0.193 0.406 0.726 1.465 1.897 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.014 0.051 0.090 0.193 0.406 0.726 1.465 1.897 O.D. 0.014 0.051 0.090 0.193 0.406 0.726 1.465 1.897

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.