Human R-Spondin1 PicoKine® Quick ELISA Kit

Human R-Spondin-1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human RSPO1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

R-spondin-1 is a secreted protein that in humans is encoded by the Rspo1 gene. It is mapped to chromosome 1p34. This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy- induced adverse effects. Alternative splicing results in multiple transcript variants.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 102 90 89 99 95 5.61 5.9% Sample 2 315 298 275 295 295 14.2 4.8% Sample 3 845 829 877 951 875 46.89 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 102 90 89 99 95 5.61 5.9% Sample 1 102 90 89 99 95 5.61 5.9% Sample 2 315 298 275 295 295 14.2 4.8% Sample 2 315 298 275 295 295 14.2 4.8% Sample 3 845 829 877 951 875 46.89 5.3% Sample 3 845 829 877 951 875 46.89 5.3%

Synonyms

R-spondin-1; Roof plate-specific spondin-1; hRspo1; RSPO1

Gene Name

RSPO1

Gene ID

284654

UniProt

Q2MKA7

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S21-A263

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 102 315 845 98 304 805 Standard deviation 5.60 16.06 42.25 6.46 16.41 45.08 CV (%) 5.6% 5.6% 5% 6.6% 5.4% 5.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 102 315 845 98 304 805 Standard deviation 5.60 16.06 42.25 6.46 16.41 45.08 CV (%) 5.6% 5.6% 5% 6.6% 5.4% 5.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 102 315 845 98 304 805 Mean (pg/ml) 102 315 845 98 304 805 Standard deviation 5.60 16.06 42.25 6.46 16.41 45.08 Standard deviation 5.60 16.06 42.25 6.46 16.41 45.08 CV (%) 5.6% 5.6% 5% 6.6% 5.4% 5.6% CV (%) 5.6% 5.6% 5% 6.6% 5.4% 5.6%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

28959 MW

Specificity

Natural and recombinant human R-Spondin-1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

R-spondin-1

Protein Name

R-spondin-1

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.030 0.063 0.097 0.158 0.308 0.653 1.412 2.031 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.030 0.063 0.097 0.158 0.308 0.653 1.412 2.031 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.030 0.063 0.097 0.158 0.308 0.653 1.412 2.031 O.D. 0.030 0.063 0.097 0.158 0.308 0.653 1.412 2.031

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.