Human CCL16/HCC-4 PicoKine® Quick ELISA Kit

Chemokine (C-C motif) ligand 16 (CCL16) is a small cytokine belonging to the CC chemokine family that is known under several pseudonyms, including Liver-expressed chemokine (LEC) and Monotactin-1 (MTN-1) . This chemokine is expressed by the liver, thymus, and spleen and is chemoattractive for monocytes and lymphocytes. Cellular expression of CCL16 can be strongly induced in monocytes by IL-10, IFN-gamma and bacterial lipopolysaccharide. Its gene is located on chromosome 17, in humans, among a cluster of other CC chemokines. CCL16 elicits its effects on cells by interacting with cell surface chemokine receptors such as CCR1, CCR2, CCR5 and CCR8.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 17 16 17 17 16 0.43 2.6% Sample 2 60 53 52 58 55 3.34 6% Sample 3 271 227 243 230 242 17.38 7.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 17 16 17 17 16 0.43 2.6% Sample 1 17 16 17 17 16 0.43 2.6% Sample 2 60 53 52 58 55 3.34 6% Sample 2 60 53 52 58 55 3.34 6% Sample 3 271 227 243 230 242 17.38 7.1% Sample 3 271 227 243 230 242 17.38 7.1%

C-C motif chemokine 16; Chemokine CC-4; HCC-4; Chemokine LEC; IL-10-inducible chemokine; LCC-1; Liver-expressed chemokine; Lymphocyte and monocyte chemoattractant; LMC; Monotactin-1; MTN-1; NCC-4; Small-inducible cytokine A16; CCL16; ILINCK, NCC4, SCYA16

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 17 60 271 18 54 267 Standard deviation 0.85 3.18 20.86 1.02 3.56 21.36 CV (%) 5% 5.3% 7.7% 5.7% 6.6% 8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 17 60 271 18 54 267 Standard deviation 0.85 3.18 20.86 1.02 3.56 21.36 CV (%) 5% 5.3% 7.7% 5.7% 6.6% 8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 17 60 271 18 54 267 Mean (pg/ml) 17 60 271 18 54 267 Standard deviation 0.85 3.18 20.86 1.02 3.56 21.36 Standard deviation 0.85 3.18 20.86 1.02 3.56 21.36 CV (%) 5% 5.3% 7.7% 5.7% 6.6% 8% CV (%) 5% 5.3% 7.7% 5.7% 6.6% 8%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.024 0.070 0.117 0.200 0.384 0.777 1.422 2.095 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.024 0.070 0.117 0.200 0.384 0.777 1.422 2.095 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.024 0.070 0.117 0.200 0.384 0.777 1.422 2.095 O.D. 0.024 0.070 0.117 0.200 0.384 0.777 1.422 2.095

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.