Human CCL16/HCC-4 PicoKine® Quick ELISA Kit

Human CCL16/HCC-4 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human CCL16 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chemokine (C-C motif) ligand 16 (CCL16) is a small cytokine belonging to the CC chemokine family that is known under several pseudonyms, including Liver-expressed chemokine (LEC) and Monotactin-1 (MTN-1) . This chemokine is expressed by the liver, thymus, and spleen and is chemoattractive for monocytes and lymphocytes. Cellular expression of CCL16 can be strongly induced in monocytes by IL-10, IFN-gamma and bacterial lipopolysaccharide. Its gene is located on chromosome 17, in humans, among a cluster of other CC chemokines. CCL16 elicits its effects on cells by interacting with cell surface chemokine receptors such as CCR1, CCR2, CCR5 and CCR8.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 17 16 17 17 16 0.43 2.6% Sample 2 60 53 52 58 55 3.34 6% Sample 3 271 227 243 230 242 17.38 7.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 17 16 17 17 16 0.43 2.6% Sample 1 17 16 17 17 16 0.43 2.6% Sample 2 60 53 52 58 55 3.34 6% Sample 2 60 53 52 58 55 3.34 6% Sample 3 271 227 243 230 242 17.38 7.1% Sample 3 271 227 243 230 242 17.38 7.1%

Synonyms

C-C motif chemokine 16; Chemokine CC-4; HCC-4; Chemokine LEC; IL-10-inducible chemokine; LCC-1; Liver-expressed chemokine; Lymphocyte and monocyte chemoattractant; LMC; Monotactin-1; MTN-1; NCC-4; Small-inducible cytokine A16; CCL16; ILINCK, NCC4, SCYA16

Gene Name

CCL16

Gene ID

6360

UniProt

O15467

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Q24-Q120

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

7.8 pg/mL - 500 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 17 60 271 18 54 267 Standard deviation 0.85 3.18 20.86 1.02 3.56 21.36 CV (%) 5% 5.3% 7.7% 5.7% 6.6% 8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 17 60 271 18 54 267 Standard deviation 0.85 3.18 20.86 1.02 3.56 21.36 CV (%) 5% 5.3% 7.7% 5.7% 6.6% 8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 17 60 271 18 54 267 Mean (pg/ml) 17 60 271 18 54 267 Standard deviation 0.85 3.18 20.86 1.02 3.56 21.36 Standard deviation 0.85 3.18 20.86 1.02 3.56 21.36 CV (%) 5% 5.3% 7.7% 5.7% 6.6% 8% CV (%) 5% 5.3% 7.7% 5.7% 6.6% 8%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

13600 MW

Specificity

Natural and recombinant human CCL16

Anticoagulant

Heparin or EDTA

Gene Name Synonym

C-C motif chemokine 16

Protein Name

C-C motif chemokine 16

Sample Data

Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.024 0.070 0.117 0.200 0.384 0.777 1.422 2.095 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.024 0.070 0.117 0.200 0.384 0.777 1.422 2.095 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.024 0.070 0.117 0.200 0.384 0.777 1.422 2.095 O.D. 0.024 0.070 0.117 0.200 0.384 0.777 1.422 2.095

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.