Human Cathepsin V PicoKine® Quick ELISA Kit

Human Cathepsin V PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human CTSV in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Cathepsin L2, also known as cathepsin V and encoded by the CTSL2 gene, is a human gene. It is mapped to 9q22.33. The protein encoded by this gene, a member of the peptidase C1 family, is a lysosomal cysteine proteinase that may play an important role in corneal physiology. This gene is expressed in colorectal and breast carcinomas but not in normal colon, mammary gland, or peritumoral tissues, suggesting a possible role for this gene in tumor processes. Alternatively spliced variants, encoding the same protein, have been identified.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 100 121 111 116 112 7.77 6.9% Sample 2 741 756 857 851 801 53.05 6.6% Sample 3 2346 2384 2464 2369 2390 44.40 1.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 100 121 111 116 112 7.77 6.9% Sample 1 100 121 111 116 112 7.77 6.9% Sample 2 741 756 857 851 801 53.05 6.6% Sample 2 741 756 857 851 801 53.05 6.6% Sample 3 2346 2384 2464 2369 2390 44.40 1.8% Sample 3 2346 2384 2464 2369 2390 44.40 1.8%

Synonyms

Cathepsin L2; Cathepsin U; Cathepsin V; CTSV; CATL2; CTSL2; CTSU; UNQ268/PRO305

Gene Name

CTSV

Gene ID

1515

UniProt

O60911

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: V18-V334

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

78 pg/mL - 5,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 100 714 2346 136 823 2464 Standard deviation 5.30 44.27 105.57 6.39 52.67 133.06 CV (%) 5.3% 6.2% 4.5% 4.7% 6.4% 5.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 100 714 2346 136 823 2464 Standard deviation 5.30 44.27 105.57 6.39 52.67 133.06 CV (%) 5.3% 6.2% 4.5% 4.7% 6.4% 5.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 100 714 2346 136 823 2464 Mean (pg/ml) 100 714 2346 136 823 2464 Standard deviation 5.30 44.27 105.57 6.39 52.67 133.06 Standard deviation 5.30 44.27 105.57 6.39 52.67 133.06 CV (%) 5.3% 6.2% 4.5% 4.7% 6.4% 5.4% CV (%) 5.3% 6.2% 4.5% 4.7% 6.4% 5.4%

Sensitivity

<15 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant human Cathepsin V

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Cathepsin V

Sample Data

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.096 0.132 0.216 0.379 0.609 1.011 1.828 2.542 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.096 0.132 0.216 0.379 0.609 1.011 1.828 2.542 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.096 0.132 0.216 0.379 0.609 1.011 1.828 2.542 O.D. 0.096 0.132 0.216 0.379 0.609 1.011 1.828 2.542

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.