Human CD97 PicoKine® Quick ELISA Kit

Cluster of differentiation 97 is a protein also known as BL-Ac [F2] encoded by the ADGRE5 gene. The CD97 gene is mapped to 19p13.2-p13.12 by fluorescence in situ hybridization. In the immune system, CD97 is known as a critical mediator of host defense. Upon lymphoid, myeloid cells and neutrophil activation, CD97 is upregulated to promote adhesion and migration to sites of inflammation. Moreover, it has been shown that CD97 regulates granulocyte homeostasis. CD97 is expressed at the sarcoplasmic reticulum and the peripheral sarcolemma in skeletal muscle. However, lack of CD97 only affects the structure of the sarcoplasmic reticulum, but not the function of skeletal muscle.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 192 169 180 190 182 9.14 5% Sample 2 1210 1172 1113 1111 1151 41.72 3.6% Sample 3 5765 6054 5419 5224 5615 318.79 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 192 169 180 190 182 9.14 5% Sample 1 192 169 180 190 182 9.14 5% Sample 2 1210 1172 1113 1111 1151 41.72 3.6% Sample 2 1210 1172 1113 1111 1151 41.72 3.6% Sample 3 5765 6054 5419 5224 5615 318.79 5.6% Sample 3 5765 6054 5419 5224 5615 318.79 5.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 192 1210 5765 193 1108 6274 Standard deviation 12.09 62.92 253.66 13.12 77.56 288.6 CV (%) 7% 5.2% 4.4% 6.8% 7% 4.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 192 1210 5765 193 1108 6274 Standard deviation 12.09 62.92 253.66 13.12 77.56 288.6 CV (%) 7% 5.2% 4.4% 6.8% 7% 4.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 192 1210 5765 193 1108 6274 Mean (pg/ml) 192 1210 5765 193 1108 6274 Standard deviation 12.09 62.92 253.66 13.12 77.56 288.6 Standard deviation 12.09 62.92 253.66 13.12 77.56 288.6 CV (%) 7% 5.2% 4.4% 6.8% 7% 4.6% CV (%) 7% 5.2% 4.4% 6.8% 7% 4.6%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.004 0.102 0.175 0.333 0.559 1.021 1.672 2.054 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.004 0.102 0.175 0.333 0.559 1.021 1.672 2.054 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.004 0.102 0.175 0.333 0.559 1.021 1.672 2.054 O.D. 0.004 0.102 0.175 0.333 0.559 1.021 1.672 2.054

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.