Mouse CD6 PicoKine® Quick ELISA Kit

Mouse soluble CD6 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Mouse Cd6 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CD6 (Cluster of Differentiation 6) is a human protein encoded by the CD6 gene. It is mapped to 11q12.2. CD6 is expressed predominantly in peripheral T cells and mature medullary thymocytes. This gene encodes a protein found on the outer membrane of T-lymphocytes as well as some other immune cells. The encoded protein contains three scavenger receptor cysteine-rich (SRCR) domains and a binding site for an activated leukocyte cell adhesion molecule. The gene product is important for continuation of T cell activation. What’s more, this gene may be associated with susceptibility to multiple sclerosis.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 133 133 120 119 126 6.75 5.3% Sample 2 525 507 540 469 510 26.52 5.2% Sample 3 1268 1267 1220 1339 1273 42.5 3.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 133 133 120 119 126 6.75 5.3% Sample 1 133 133 120 119 126 6.75 5.3% Sample 2 525 507 540 469 510 26.52 5.2% Sample 2 525 507 540 469 510 26.52 5.2% Sample 3 1268 1267 1220 1339 1273 42.5 3.3% Sample 3 1268 1267 1220 1339 1273 42.5 3.3%

Synonyms

T-cell differentiation antigen CD6; CD6; Cd6

Gene Name

CD6

Gene ID

12511

UniProt

Q61003

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L18-G396

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 133 525 1268 129 485 1288 Standard deviation 5.58 38.9%7 92.56 6.45 37.83 114.63 CV (%) 4.2% 6.7% 7.3% 5% 7.8% 8.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 133 525 1268 129 485 1288 Standard deviation 5.58 38.9%7 92.56 6.45 37.83 114.63 CV (%) 4.2% 6.7% 7.3% 5% 7.8% 8.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 133 525 1268 129 485 1288 Mean (pg/ml) 133 525 1268 129 485 1288 Standard deviation 5.58 38.9%7 92.56 6.45 37.83 114.63 Standard deviation 5.58 38.9%7 92.56 6.45 37.83 114.63 CV (%) 4.2% 6.7% 7.3% 5% 7.8% 8.9% CV (%) 4.2% 6.7% 7.3% 5% 7.8% 8.9%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

72255 MW

Specificity

Natural and recombinant mouse CD6

Anticoagulant

Heparin or EDTA

Gene Name Synonym

T-cell differentiation antigen CD6

Protein Name

T-cell differentiation antigen CD6

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.014 0.059 0.1 0.185 0.349 0.666 1.217 2.030 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.014 0.059 0.1 0.185 0.349 0.666 1.217 2.030 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.014 0.059 0.1 0.185 0.349 0.666 1.217 2.030 O.D. 0.014 0.059 0.1 0.185 0.349 0.666 1.217 2.030

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.