Human caspase3 PicoKine® Quick ELISA Kit

Human Caspase 3/CASP3 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human CASP3 in cell culture supernatant sand serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Caspase-3 is a caspase protein that is encoded by the CASP3 gene. This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 63 54 63 61 60 3.69 6.1% Sample 2 211 224 236 237 227 10.55 4.6% Sample 3 945 874 867 995 920 52.85 5.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 63 54 63 61 60 3.69 6.1% Sample 1 63 54 63 61 60 3.69 6.1% Sample 2 211 224 236 237 227 10.55 4.6% Sample 2 211 224 236 237 227 10.55 4.6% Sample 3 945 874 867 995 920 52.85 5.7% Sample 3 945 874 867 995 920 52.85 5.7%

Synonyms

Caspase-3; CASP-3;3.4.22.56; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1; Caspase-3 subunit p17; Caspase-3 subunit p12; CASP3; CPP32

Gene Name

CASP3

Gene ID

836

UniProt

P42574

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S29-D175 & A183-H277

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatant sand serum.

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 63 211 945 69 203 995 Standard deviation 3.27 15.4 55.75 4.2 17.86 62.68 CV (%) 5.2% 7.3% 5.9% 6.1% 8.8% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 63 211 945 69 203 995 Standard deviation 3.27 15.4 55.75 4.2 17.86 62.68 CV (%) 5.2% 7.3% 5.9% 6.1% 8.8% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 63 211 945 69 203 995 Mean (pg/ml) 63 211 945 69 203 995 Standard deviation 3.27 15.4 55.75 4.2 17.86 62.68 Standard deviation 3.27 15.4 55.75 4.2 17.86 62.68 CV (%) 5.2% 7.3% 5.9% 6.1% 8.8% 6.3% CV (%) 5.2% 7.3% 5.9% 6.1% 8.8% 6.3%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

31608 MW

Specificity

Natural and recombinant human Caspase 3

Gene Name Synonym

Caspase-3

Protein Name

Caspase-3

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.001 0.041 0.084 0.168 0.262 0.476 0.984 1.886 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.001 0.041 0.084 0.168 0.262 0.476 0.984 1.886 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.001 0.041 0.084 0.168 0.262 0.476 0.984 1.886 O.D. 0.001 0.041 0.084 0.168 0.262 0.476 0.984 1.886

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.