Human Biglycan PicoKine® Quick ELISA Kit

Biglycan (BGN) is a small leucine-rich repeat proteoglycan (SLRP) which is found in a variety of extracellular matrix tissues, including bone, cartilage and tendon. It is an important structural component of articular cartilage and participates in the assembly of the chondrocyte extracellular matrix through formation of protein interactions with type VI collagen and large proteoglycan aggregates. In humans, Biglycan is encoded by the BGN gene. It is mapped to Xq28. Biglycan is believed to play a role in the mineralisation of bone, and its core protein binds to the growth factors BMP-4 and influences its bioactivity. It has been reported that the presence of biglycan is necessary for BMP-4 to exert its effects on osteoblasts. There is also evidence that biglycan can bind to TGF-beta 1. In addition, Biglycan plays an important role in cell signaling.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 190 188 215 183 194 12.38 6.3% Sample 2 939 960 955 1125 994 75.59 7.6% Sample 3 4176 4429 4400 3916 4230 206.16 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 190 188 215 183 194 12.38 6.3% Sample 1 190 188 215 183 194 12.38 6.3% Sample 2 939 960 955 1125 994 75.59 7.6% Sample 2 939 960 955 1125 994 75.59 7.6% Sample 3 4176 4429 4400 3916 4230 206.16 4.8% Sample 3 4176 4429 4400 3916 4230 206.16 4.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 190 939 4176 179 981 4439 Standard deviation 9.12 54.46 167.04 10.2 56.89 217.51 CV (%) 4.8% 5.8% 4% 5.7% 5.8% 4.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 190 939 4176 179 981 4439 Standard deviation 9.12 54.46 167.04 10.2 56.89 217.51 CV (%) 4.8% 5.8% 4% 5.7% 5.8% 4.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 190 939 4176 179 981 4439 Mean (pg/ml) 190 939 4176 179 981 4439 Standard deviation 9.12 54.46 167.04 10.2 56.89 217.51 Standard deviation 9.12 54.46 167.04 10.2 56.89 217.51 CV (%) 4.8% 5.8% 4% 5.7% 5.8% 4.9% CV (%) 4.8% 5.8% 4% 5.7% 5.8% 4.9%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.007 0.070 0.128 0.240 0.471 0.828 1.465 2.066 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.007 0.070 0.128 0.240 0.471 0.828 1.465 2.066 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.007 0.070 0.128 0.240 0.471 0.828 1.465 2.066 O.D. 0.007 0.070 0.128 0.240 0.471 0.828 1.465 2.066

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.