Human Biglycan PicoKine® Quick ELISA Kit

Human Biglycan PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human BGN in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Biglycan (BGN) is a small leucine-rich repeat proteoglycan (SLRP) which is found in a variety of extracellular matrix tissues, including bone, cartilage and tendon. It is an important structural component of articular cartilage and participates in the assembly of the chondrocyte extracellular matrix through formation of protein interactions with type VI collagen and large proteoglycan aggregates. In humans, Biglycan is encoded by the BGN gene. It is mapped to Xq28. Biglycan is believed to play a role in the mineralisation of bone, and its core protein binds to the growth factors BMP-4 and influences its bioactivity. It has been reported that the presence of biglycan is necessary for BMP-4 to exert its effects on osteoblasts. There is also evidence that biglycan can bind to TGF-beta 1. In addition, Biglycan plays an important role in cell signaling.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 190 188 215 183 194 12.38 6.3% Sample 2 939 960 955 1125 994 75.59 7.6% Sample 3 4176 4429 4400 3916 4230 206.16 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 190 188 215 183 194 12.38 6.3% Sample 1 190 188 215 183 194 12.38 6.3% Sample 2 939 960 955 1125 994 75.59 7.6% Sample 2 939 960 955 1125 994 75.59 7.6% Sample 3 4176 4429 4400 3916 4230 206.16 4.8% Sample 3 4176 4429 4400 3916 4230 206.16 4.8%

Synonyms

Biglycan; Bone/cartilage proteoglycan I; PG-S1; BGN; SLRR1A

Gene Name

BGN

Gene ID

633

UniProt

P21810

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D38-K368

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 190 939 4176 179 981 4439 Standard deviation 9.12 54.46 167.04 10.2 56.89 217.51 CV (%) 4.8% 5.8% 4% 5.7% 5.8% 4.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 190 939 4176 179 981 4439 Standard deviation 9.12 54.46 167.04 10.2 56.89 217.51 CV (%) 4.8% 5.8% 4% 5.7% 5.8% 4.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 190 939 4176 179 981 4439 Mean (pg/ml) 190 939 4176 179 981 4439 Standard deviation 9.12 54.46 167.04 10.2 56.89 217.51 Standard deviation 9.12 54.46 167.04 10.2 56.89 217.51 CV (%) 4.8% 5.8% 4% 5.7% 5.8% 4.9% CV (%) 4.8% 5.8% 4% 5.7% 5.8% 4.9%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

41654 MW

Specificity

Natural and recombinant human Biglycan

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Biglycan

Protein Name

Biglycan

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.007 0.070 0.128 0.240 0.471 0.828 1.465 2.066 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.007 0.070 0.128 0.240 0.471 0.828 1.465 2.066 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.007 0.070 0.128 0.240 0.471 0.828 1.465 2.066 O.D. 0.007 0.070 0.128 0.240 0.471 0.828 1.465 2.066

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.