Human IL-2 PicoKine® Quick ELISA Kit

Human IL-2/Interleukin-2 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human IL2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. The human gene for interleukin 2 (IL2) is assigned to chromosome 4. Human IL-2 is a 15.5KDa glycoprotein, consisting of 153 amino acids in precursor form and 133 amino acids in mature form.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 29 28 30 29 1.11 3.8% Sample 2 158 160 151 163 158 4.41 2.7% Sample 3 484 469 488 480 480 7.08 1.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 29 28 30 29 1.11 3.8% Sample 1 31 29 28 30 29 1.11 3.8% Sample 2 158 160 151 163 158 4.41 2.7% Sample 2 158 160 151 163 158 4.41 2.7% Sample 3 484 469 488 480 480 7.08 1.4% Sample 3 484 469 488 480 480 7.08 1.4%

Synonyms

Interleukin-2; IL-2; T-cell growth factor; TCGF; Aldesleukin; IL2

Gene Name

IL2

Gene ID

3558

UniProt

P60568

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A21-T153

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 158 484 28 169 474 Standard deviation 1.27 8.84 30 1.14 10.98 38.39 CV (%) 4.1% 5.6% 6.2% 4.1% 6.5% 8.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 158 484 28 169 474 Standard deviation 1.27 8.84 30 1.14 10.98 38.39 CV (%) 4.1% 5.6% 6.2% 4.1% 6.5% 8.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 31 158 484 28 169 474 Mean (pg/ml) 31 158 484 28 169 474 Standard deviation 1.27 8.84 30 1.14 10.98 38.39 Standard deviation 1.27 8.84 30 1.14 10.98 38.39 CV (%) 4.1% 5.6% 6.2% 4.1% 6.5% 8.1% CV (%) 4.1% 5.6% 6.2% 4.1% 6.5% 8.1%

Sensitivity

<1 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

17628 MW

Specificity

Natural and recombinant human IL-2

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Interleukin-2

Protein Name

Interleukin-2

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.030 0.065 0.123 0.212 0.336 0.609 1.031 1.798 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.030 0.065 0.123 0.212 0.336 0.609 1.031 1.798 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.030 0.065 0.123 0.212 0.336 0.609 1.031 1.798 O.D. 0.030 0.065 0.123 0.212 0.336 0.609 1.031 1.798

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.