Human ADAM15 PicoKine® Quick ELISA Kit

Human ADAM15 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human ADAM15 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 12pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Disintegrin and metalloproteinase domain-containing protein 15 is an enzyme that in humans is encoded by the ADAM15 gene. The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as an EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 112 107 104 107 107 2.87 2.6% Sample 2 674 753 761 757 736 36.05 4.8% Sample 3 1454 1384 1331 1442 1402 49.15 3.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 112 107 104 107 107 2.87 2.6% Sample 1 112 107 104 107 107 2.87 2.6% Sample 2 674 753 761 757 736 36.05 4.8% Sample 2 674 753 761 757 736 36.05 4.8% Sample 3 1454 1384 1331 1442 1402 49.15 3.5% Sample 3 1454 1384 1331 1442 1402 49.15 3.5%

Synonyms

Disintegrin and metalloproteinase domain-containing protein 15; ADAM 15;3.4.24.-; Metalloprotease RGD disintegrin protein; Metalloproteinase-like, disintegrin-like, and cysteine-rich protein 15; MDC-15; Metargidin; ADAM15; MDC15

Gene Name

ADAM15

Gene ID

8751

UniProt

Q13444

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D207-T696

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 112 674 1454 110 704 1511 Standard deviation 5.04 53.24 59.61 7.04 64.06 64.97 CV (%) 4.5% 7.9% 4.1% 6.4% 9.1% 4.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 112 674 1454 110 704 1511 Standard deviation 5.04 53.24 59.61 7.04 64.06 64.97 CV (%) 4.5% 7.9% 4.1% 6.4% 9.1% 4.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 112 674 1454 110 704 1511 Mean (pg/ml) 112 674 1454 110 704 1511 Standard deviation 5.04 53.24 59.61 7.04 64.06 64.97 Standard deviation 5.04 53.24 59.61 7.04 64.06 64.97 CV (%) 4.5% 7.9% 4.1% 6.4% 9.1% 4.3% CV (%) 4.5% 7.9% 4.1% 6.4% 9.1% 4.3%

Sensitivity

<12 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

92959 MW

Specificity

Natural and recombinant human ADAM15

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Disintegrin and metalloproteinase domain-containing protein 15

Protein Name

Disintegrin and metalloproteinase domain-containing protein 15

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.061 0.083 0.109 0.185 0.404 0.664 1.373 2.079 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.061 0.083 0.109 0.185 0.404 0.664 1.373 2.079 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.061 0.083 0.109 0.185 0.404 0.664 1.373 2.079 O.D. 0.061 0.083 0.109 0.185 0.404 0.664 1.373 2.079

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.