Human IL-1β PicoKine® Quick ELISA Kit

Human IL-1 Beta/IL-1F2/IL1B PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human IL1B in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 0.15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB) -regulated genes. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 11 10 11 11 10 0.43 4.3% Sample 2 60 54 52 53 54 3.11 5.7% Sample 3 123 119 123 112 119 4.49 3.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 11 10 11 11 10 0.43 4.3% Sample 1 11 10 11 11 10 0.43 4.3% Sample 2 60 54 52 53 54 3.11 5.7% Sample 2 60 54 52 53 54 3.11 5.7% Sample 3 123 119 123 112 119 4.49 3.7% Sample 3 123 119 123 112 119 4.49 3.7%

Synonyms

Interleukin-1 beta; IL-1 beta; Catabolin; IL1B; IL1F2

Gene Name

IL1B

Gene ID

3553

UniProt

P01584

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A117-S269

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated monoclonal antibody from mouse

Applications

ELISA

Standard

1 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

3.9 pg/mL - 250 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 11 60 123 10 58 112 Standard deviation 0.45 2.88 8.97 0.57 3.24 9.96 CV (%) 4.1% 4.8% 7.3% 5.7% 5.6% 8.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 11 60 123 10 58 112 Standard deviation 0.45 2.88 8.97 0.57 3.24 9.96 CV (%) 4.1% 4.8% 7.3% 5.7% 5.6% 8.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 11 60 123 10 58 112 Mean (pg/ml) 11 60 123 10 58 112 Standard deviation 0.45 2.88 8.97 0.57 3.24 9.96 Standard deviation 0.45 2.88 8.97 0.57 3.24 9.96 CV (%) 4.1% 4.8% 7.3% 5.7% 5.6% 8.9% CV (%) 4.1% 4.8% 7.3% 5.7% 5.6% 8.9%

Sensitivity

<0.15 pg/mL

Components

Description|Quantity|Volume Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 HRP-Linked Antibody|1|6ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

30748 MW

Specificity

Natural and recombinant human IL-1 beta

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Interleukin-1 beta

Protein Name

Interleukin-1 beta

Sample Data

Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 O.D. 0.022 0.074 0.105 0.249 0.423 0.707 1.095 1.830 Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 O.D. 0.022 0.074 0.105 0.249 0.423 0.707 1.095 1.830 Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 O.D. 0.022 0.074 0.105 0.249 0.423 0.707 1.095 1.830 O.D. 0.022 0.074 0.105 0.249 0.423 0.707 1.095 1.830

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.