Human ANGPTL2 PicoKine® Quick ELISA Kit

Human ANGPTL2 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human ANGPTL2 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 50 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Angiopoietin-related protein 2 also known as angiopoietin-like protein 2 is a protein that in humans is encoded by the ANGPTL2 gene. This gene is mapped to 9q33.3. Angiopoietin-like protein 2 maintains tissue homeostasis by promoting adaptive inflammation and subsequent tissue reconstruction, whereas an excess of ANGPTL2 activation induced by prolonged stress promotes the breakdown of tissue homeostasis due to chronic inflammation, promoting the development of metabolic diseases. ANGPTL2 has a role also in angiogenesis, in tissue repair, in obesity, in atherosclerotic diseases and finally in carcinogenesis. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. Angiopoietin-1, angiopoietin-2, and angiopoietin-4 participate in the formation of blood vessels. ANGPTL2 protein is a secreted glycoprotein with homology to the angiopoietins and may exert a function on endothelial cells through autocrine or paracrine action.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 590 572 610 690 615 45.06 7.3% Sample 2 1670 1703 1941 1771 1771 104.55 5.9% Sample 3 7933 8323 9098 7778 8283 510.72 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 590 572 610 690 615 45.06 7.3% Sample 1 590 572 610 690 615 45.06 7.3% Sample 2 1670 1703 1941 1771 1771 104.55 5.9% Sample 2 1670 1703 1941 1771 1771 104.55 5.9% Sample 3 7933 8323 9098 7778 8283 510.72 6.1% Sample 3 7933 8323 9098 7778 8283 510.72 6.1%

Synonyms

Angiopoietin-related protein 2; Angiopoietin-like protein 2

Gene Name

ANGPTL2

Gene ID

23452

UniProt

Q9UKU9

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q23-H493

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 590 1670 7933 594 1958 8471 Standard deviation 30.68 71.81 325.25 39.20 113.56 559.09 CV (%) 5.2% 4.3% 4.1% 6.6% 5.8% 6.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 590 1670 7933 594 1958 8471 Standard deviation 30.68 71.81 325.25 39.20 113.56 559.09 CV (%) 5.2% 4.3% 4.1% 6.6% 5.8% 6.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 590 1670 7933 594 1958 8471 Mean (pg/ml) 590 1670 7933 594 1958 8471 Standard deviation 30.68 71.81 325.25 39.20 113.56 559.09 Standard deviation 30.68 71.81 325.25 39.20 113.56 559.09 CV (%) 5.2% 4.3% 4.1% 6.6% 5.8% 6.6% CV (%) 5.2% 4.3% 4.1% 6.6% 5.8% 6.6%

Sensitivity

<50 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant human ANGPTL2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Angiopoietin like 2

Protein Name

Angiopoietin-related protein 2

Sample Data

Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.036 0.089 0.122 0.214 0.404 0.712 1.255 1.968 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.036 0.089 0.122 0.214 0.404 0.712 1.255 1.968 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.036 0.089 0.122 0.214 0.404 0.712 1.255 1.968 O.D. 0.036 0.089 0.122 0.214 0.404 0.712 1.255 1.968

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.