Human SIGLEC-1/CD169 PicoKine® Quick ELISA Kit

Human CD169/SIGLEC-1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human SIGLEC1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

SIGLEC-1, also known as Sialoadhesin or CD169, is a cell adhesion molecule found on the surface of certain cells of the immune system called macrophages. This gene is mapped to 20p13. It belongs to the immunoglobulin superfamily (IgSF) . Since sialoadhesin binds sialic acids with its N-terminal IgV-domain, it is also a member of the SIGLEC family. The localization and expression of SIGLEC-1 in humans has altered coincident with the evolutionary loss of Neu5Gc. SIGLEC-1-positive macrophages have a dual physiologic function. They act as innate 'flypaper' by preventing the systemic spread of lymph-borne pathogens and as critical gatekeepers at the lymph-tissue interface that facilitate the recognition of particulate antigens by B cells and initiate humoral immune responses.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 278 276 287 263 276 8.57 3.1% Sample 2 1821 2107 2081 1805 1953 140.91 7.2% Sample 3 4073 4478 4741 4142 4358 268.77 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 278 276 287 263 276 8.57 3.1% Sample 1 278 276 287 263 276 8.57 3.1% Sample 2 1821 2107 2081 1805 1953 140.91 7.2% Sample 2 1821 2107 2081 1805 1953 140.91 7.2% Sample 3 4073 4478 4741 4142 4358 268.77 6.1% Sample 3 4073 4478 4741 4142 4358 268.77 6.1%

Synonyms

Sialoadhesin; Sialic acid-binding Ig-like lectin 1; Siglec-1; CD169; SIGLEC1; SN

Gene Name

SIGLEC1

Gene ID

6614

UniProt

Q9BZZ2

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S20-Q1641

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 278 1821 4073 286 1650 3922 Standard deviation 20.01 138.39 240.3 22.59 148.5 262.77 CV (%) 7.2% 7.6% 5.9% 7.9% 9% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 278 1821 4073 286 1650 3922 Standard deviation 20.01 138.39 240.3 22.59 148.5 262.77 CV (%) 7.2% 7.6% 5.9% 7.9% 9% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 278 1821 4073 286 1650 3922 Mean (pg/ml) 278 1821 4073 286 1650 3922 Standard deviation 20.01 138.39 240.3 22.59 148.5 262.77 Standard deviation 20.01 138.39 240.3 22.59 148.5 262.77 CV (%) 7.2% 7.6% 5.9% 7.9% 9% 6.7% CV (%) 7.2% 7.6% 5.9% 7.9% 9% 6.7%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

182624 MW

Specificity

Natural and recombinant human CD169

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Sialoadhesin

Protein Name

Sialoadhesin

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.085 0.159 0.191 0.268 0.494 0.754 1.313 2.051 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.085 0.159 0.191 0.268 0.494 0.754 1.313 2.051 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.085 0.159 0.191 0.268 0.494 0.754 1.313 2.051 O.D. 0.085 0.159 0.191 0.268 0.494 0.754 1.313 2.051

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.