Human CXCL16 PicoKine® Quick ELISA Kit

Chemokine (C-X-C motif) ligand 16 (CXCL16) is a small cytokine belonging to the CXC chemokine family. Larger than other chemokines (with 254 amino acids), CXCL16 is composed of a CXC chemokine domain, a mucin-like stalk, a transmembrane domain and a cytoplasmic tail containing a potential tyrosine phosphorylation site that may bind SH2. These are unusual features for a chemokine, and allow CXCL16 to be expressed as a cell surface bound molecule, as well as a soluble chemokine. CXCL16 is produced by dendritic cells found in the T cell zones of lymphoid organs, and by cells found in the red pulp of the spleen. Cells that bind and migrate in response to CXCL16 include several subsets of T cells, and natural killer T (NKT) cells. CXCL16 interacts with the chemokine receptorCXCR6, also known as Bonzo. The novel CXC-chemokine ligand 16 (CXCL16) functions as transmembrane adhesion molecule on the surface of APCs and as a soluble chemoattractant for activated T cells. Expression of CXCL16 is induced by the inflammatory cytokines IFN-gamma and TNF-alpha. The gene for human CXCL16 is located on chromosome 17.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 122 112 116 125 118 5.06 4.2% Sample 2 725 748 679 765 729 32.29 4.4% Sample 3 2351 2384 2122 2446 2325 122.47 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 122 112 116 125 118 5.06 4.2% Sample 1 122 112 116 125 118 5.06 4.2% Sample 2 725 748 679 765 729 32.29 4.4% Sample 2 725 748 679 765 729 32.29 4.4% Sample 3 2351 2384 2122 2446 2325 122.47 5.2% Sample 3 2351 2384 2122 2446 2325 122.47 5.2%

C-X-C motif chemokine 16; Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; SR-PSOX; Small-inducible cytokine B16; Transmembrane chemokine CXCL16; CXCL16; SCYB16, SRPSOX; UNQ2759/PRO6714

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 122 725 2351 133 696 2150 Standard deviation 9.15 55.82 185.72 10.24 57.7%8 197.8 CV (%) 7.5% 7.7% 7.9% 7.7% 8% 9.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 122 725 2351 133 696 2150 Standard deviation 9.15 55.82 185.72 10.24 57.7%8 197.8 CV (%) 7.5% 7.7% 7.9% 7.7% 8% 9.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 122 725 2351 133 696 2150 Mean (pg/ml) 122 725 2351 133 696 2150 Standard deviation 9.15 55.82 185.72 10.24 57.7%8 197.8 Standard deviation 9.15 55.82 185.72 10.24 57.7%8 197.8 CV (%) 7.5% 7.7% 7.9% 7.7% 8% 9.2% CV (%) 7.5% 7.7% 7.9% 7.7% 8% 9.2%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 93.7 187.5 375 750 1500 3000 6000 O.D. 0.096 0.164 0.291 0.433 0.715 1.073 1.593 2.046 Concentration (pg/ml) 0 93.7 187.5 375 750 1500 3000 6000 O.D. 0.096 0.164 0.291 0.433 0.715 1.073 1.593 2.046 Concentration (pg/ml) 0 93.7 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.7 187.5 375 750 1500 3000 6000 O.D. 0.096 0.164 0.291 0.433 0.715 1.073 1.593 2.046 O.D. 0.096 0.164 0.291 0.433 0.715 1.073 1.593 2.046

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.