Mouse EGFR PicoKine® Quick ELISA Kit

Mouse EGFR PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Mouse Egfr in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4) . EGFR exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor α (TGFα) . EGFR and its ligands are cell signaling molecules involved in diverse cellular functions, including cell proliferation, differentiation, motility, and survival, and in tissue development. Mutations that lead to EGFR overexpression (known as upregulation) or overactivity have been associated with a number of cancers, including lung cancer and glioblastoma multiforme. In this latter case a more or less specific mutation of EGFR, called EGFRvIII is often observed.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 314 365 341 349 342 18.45 5.3% Sample 2 1163 1083 1036 1179 1115 58.44 5.2% Sample 3 5358 5497 5082 4676 5153 313.42 6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 314 365 341 349 342 18.45 5.3% Sample 1 314 365 341 349 342 18.45 5.3% Sample 2 1163 1083 1036 1179 1115 58.44 5.2% Sample 2 1163 1083 1036 1179 1115 58.44 5.2% Sample 3 5358 5497 5082 4676 5153 313.42 6% Sample 3 5358 5497 5082 4676 5153 313.42 6%

Synonyms

Epidermal growth factor receptor;2.7.10.1; Egfr

Gene Name

Egfr

Gene ID

13649

UniProt

Q01279

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L25-S647

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 314 1163 5358 339 1146 5763 Standard deviation 16.95 60.47 241.11 21.01 75.2%3 305.43 CV (%) 5.4% 5.2% 4.5% 6.2% 6.6% 5.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 314 1163 5358 339 1146 5763 Standard deviation 16.95 60.47 241.11 21.01 75.2%3 305.43 CV (%) 5.4% 5.2% 4.5% 6.2% 6.6% 5.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 314 1163 5358 339 1146 5763 Mean (pg/ml) 314 1163 5358 339 1146 5763 Standard deviation 16.95 60.47 241.11 21.01 75.2%3 305.43 Standard deviation 16.95 60.47 241.11 21.01 75.2%3 305.43 CV (%) 5.4% 5.2% 4.5% 6.2% 6.6% 5.3% CV (%) 5.4% 5.2% 4.5% 6.2% 6.6% 5.3%

Sensitivity

<15 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

134853 MW

Specificity

Natural and recombinant mouse EGFR

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Epidermal growth factor receptor

Protein Name

Epidermal growth factor receptor

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.058 0.104 0.130 0.195 0.355 0.644 1.158 1.908 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.058 0.104 0.130 0.195 0.355 0.644 1.158 1.908 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.058 0.104 0.130 0.195 0.355 0.644 1.158 1.908 O.D. 0.058 0.104 0.130 0.195 0.355 0.644 1.158 1.908

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.