Mouse EGFR PicoKine® Quick ELISA Kit

The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4) . EGFR exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor α (TGFα) . EGFR and its ligands are cell signaling molecules involved in diverse cellular functions, including cell proliferation, differentiation, motility, and survival, and in tissue development. Mutations that lead to EGFR overexpression (known as upregulation) or overactivity have been associated with a number of cancers, including lung cancer and glioblastoma multiforme. In this latter case a more or less specific mutation of EGFR, called EGFRvIII is often observed.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 314 365 341 349 342 18.45 5.3% Sample 2 1163 1083 1036 1179 1115 58.44 5.2% Sample 3 5358 5497 5082 4676 5153 313.42 6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 314 365 341 349 342 18.45 5.3% Sample 1 314 365 341 349 342 18.45 5.3% Sample 2 1163 1083 1036 1179 1115 58.44 5.2% Sample 2 1163 1083 1036 1179 1115 58.44 5.2% Sample 3 5358 5497 5082 4676 5153 313.42 6% Sample 3 5358 5497 5082 4676 5153 313.42 6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 314 1163 5358 339 1146 5763 Standard deviation 16.95 60.47 241.11 21.01 75.2%3 305.43 CV (%) 5.4% 5.2% 4.5% 6.2% 6.6% 5.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 314 1163 5358 339 1146 5763 Standard deviation 16.95 60.47 241.11 21.01 75.2%3 305.43 CV (%) 5.4% 5.2% 4.5% 6.2% 6.6% 5.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 314 1163 5358 339 1146 5763 Mean (pg/ml) 314 1163 5358 339 1146 5763 Standard deviation 16.95 60.47 241.11 21.01 75.2%3 305.43 Standard deviation 16.95 60.47 241.11 21.01 75.2%3 305.43 CV (%) 5.4% 5.2% 4.5% 6.2% 6.6% 5.3% CV (%) 5.4% 5.2% 4.5% 6.2% 6.6% 5.3%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.058 0.104 0.130 0.195 0.355 0.644 1.158 1.908 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.058 0.104 0.130 0.195 0.355 0.644 1.158 1.908 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.058 0.104 0.130 0.195 0.355 0.644 1.158 1.908 O.D. 0.058 0.104 0.130 0.195 0.355 0.644 1.158 1.908

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.