Human Neurotrophin-3/NT-3 PicoKine® Quick ELISA Kit

Human Neurotrophin-3 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human NTF3 in cell culture supernatants and serum. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Neurotrophin-3 (NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system. NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3 (Ntrk3; also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest (NC) cell proliferation in vivo. NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.1

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 30 28 27 28 28 1.08 3.8% Sample 2 147 147 172 166 158 11.2 7% Sample 3 434 515 474 436 464 33.1 7.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 30 28 27 28 28 1.08 3.8% Sample 1 30 28 27 28 28 1.08 3.8% Sample 2 147 147 172 166 158 11.2 7% Sample 2 147 147 172 166 158 11.2 7% Sample 3 434 515 474 436 464 33.1 7.1% Sample 3 434 515 474 436 464 33.1 7.1%

Synonyms

Neurotrophin-3; NT-3; HDNF; Nerve growth factor 2; NGF-2; Neurotrophic factor; NTF3

Gene Name

NTF3

Gene ID

4908

UniProt

P20783

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Y139-T257

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants and serum.

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 30 147 434 31 141 418 Standard deviation 1.83 6.02 29.94 2.32 7.61 33.44 CV (%) 6.1% 4.1% 6.9% 7.5% 5.4% 8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 30 147 434 31 141 418 Standard deviation 1.83 6.02 29.94 2.32 7.61 33.44 CV (%) 6.1% 4.1% 6.9% 7.5% 5.4% 8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 30 147 434 31 141 418 Mean (pg/ml) 30 147 434 31 141 418 Standard deviation 1.83 6.02 29.94 2.32 7.61 33.44 Standard deviation 1.83 6.02 29.94 2.32 7.61 33.44 CV (%) 6.1% 4.1% 6.9% 7.5% 5.4% 8% CV (%) 6.1% 4.1% 6.9% 7.5% 5.4% 8%

Sensitivity

<2 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

29355 MW

Specificity

Natural and recombinant human Neurotrophin-3

Gene Name Synonym

Neurotrophin-3

Protein Name

Neurotrophin-3

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.014 0.065 0.173 0.248 0.477 0.761 1.270 1.886 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.014 0.065 0.173 0.248 0.477 0.761 1.270 1.886 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.014 0.065 0.173 0.248 0.477 0.761 1.270 1.886 O.D. 0.014 0.065 0.173 0.248 0.477 0.761 1.270 1.886

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.